The STEMGOLD High-Performance MSC Growth Medium kit comes with a reliable and comprehensive protocol to ensure optimal results. The protocol begins with the preparation of the complete medium, involving the mixing of STEMGOLD Growth Supplements with the STEMGOLD Basal Medium. For the revival of cryopreserved human MSCs, the protocol provides a detailed guide, suggesting a seeding density of 3 to 6 x 103 cells/cm2 in a T-75 cm2 tissue culture-treated flask. The cells are then incubated for three days to achieve 80% confluency. The protocol also covers the expansion of human MSCs, detailing the steps from warming the complete medium to achieving the desired cell density. This user-friendly protocol is designed to guide both novice and experienced researchers to ensure successful growth and expansion of human MSCs with STEMGOLD.

Here’s comparison of mesenchymal stem cells growth after 4 days of sub-culture in 3 different medium: Brand A, Brand B, STEMGOLD

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