Monoclonal Antibody Preparation Service

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Monoclonal Antibody Preparation Service

Monoclonal antibody technology involves the fusion of a single B lymphocyte, capable of producing antibodies, with myeloma cells, creating hybrid cells that can both generate antibodies and proliferate indefinitely. This process significantly boosts antibody production and has widespread applications in areas such as basic research, medical diagnostics, and tumor-targeted therapies.

At Atlantis Bioscience, we’ve partnered with Synbio Technologies to offer you access to their advanced antibody drug discovery platform, backed by a skilled scientific team. Together, we provide a full range of services in custom monoclonal antibody production, including immunization, hybridoma development, antibody discovery, functional screening assays, antibody engineering, and expression. Through this collaboration, we can deliver up to 10 high-quality, functional hybridoma clones, helping to accelerate your antibody research and drive successful outcomes.

Highlights

  • Exclusive Immunization Technology

    The Universal Antigen is capable of stimulating the production of antibodies that recognize epitopes consisting of fewer than 5 amino acids.

  • Accelerated Screening

    Immunization and high-throughput screening are optimized for measuring affinity, conducting epitope binning, isotyping, and other analyses.

  • One-Stop Solution

    The streamlined approach emphasizes immunization, hybridoma development, and characterization.

  • All-Inclusive Functional Assay

    We have extensive expertise in antibody affinity ranking, affinity measurement, cross-reactivity, and various other in-vitro biological analyses.

Service Details

MilestonesDetailsTATDeliverable
Animal ImmunizationBlood collection before immunization7-9 weeksInterim report (Including antiserum titer test results)
First immunization 
(Antigen plus Freund’s complete adjuvant)
Second immunization 
(Antigen plus Freund’s incomplete adjuvant)
Third immunization 
(Antigen plus Freund’s incomplete adjuvant)
Blood sampling and ELISA test
Fourth Immunization (Antigen is dissolved in PBS or saline)
Animal blood collection

Cell fusion and screening		Cell fusion and screening5-6 weeksInterim report (Including screening data)
Subcloning and cell expansion
Cell line cryopreservation
Antibody production and purificationHybridoma cell culture4-6 weeks1-3mg monoclonal antibody with a titer of 100,000 
Final experiment report

FAQs

What are the differences between monoclonal antibodies and polyclonal antibodies?

Monoclonal AntibodyPolyclonal Antibody
Identify a unique epitope of a specific antigenIdentify multiple epitopes of a specific antigen
Specific detection of an unique target epitope, without cross-reactions with other antigens; lower staining background interferenceMore cross-reactions with similar antigens; can amplify the signal of the target protein with low expression level; higher staining background interference
Detection of an unique antigen or a single member of a protein familyMore tolerant to small antigen changes and can detect homologous protein families
Impure immunogens can also produce high purity antibodiesHigher purity of the immunogen will obtain better polyclonal antibodies
Easy to standardize; the antibody test results from different batches are stable and consistentThe quality of antibodies varies greatly from batch to batch
Higher production cost; longer turnaround timeLower production cost; shorter turnaround time

How to choose mAb or pAb?

Monoclonal antibody applications:

  • Quantifying protein expression levels
  • Immunocytochemistry (ICC), immunohistochemistry (IHC), and immunofluorescence (IF)
  • Detecting structural changes and phosphorylation states of antigens

Polyclonal antibody applications:

  • Capturing a broad range of antigens (e.g., IP or ChIP)
  • Detecting denatured proteins
  • Identifying proteins that may have undergone glycosylation or morphological changes

What are the advantages and disadvantages of monoclonal antibodies ?

Advantages:

  • High specificity, recognizing only a single epitope of an antigen
  • Hybridoma cell lines can produce unlimited quantities of antibodies
  • Consistent results
  • Low background noise and minimal cross-reactivity
  • Ideal for affinity purification

Disadvantages:

  • Higher cost
  • Longer production timelines
  • Potentially lower signal strength
  • More susceptible to epitope loss due to chemical treatment of the antigen, though this can be mitigated by pooling multiple monoclonal antibodies

Resources

Gene Synthesis Overview
Recombinant Antibody Production

Ready to take your antibody research and production to the next level? Get in touch with us below to discuss your needs and request a customized quote. Let’s accelerate your research and deliver the high-quality results together!

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