To support preclinical in-vivo research and development, GemPharmatech has developed a comprehensive platform for gene-edited cell line customization, reporter gene cell line customization, in-vivo mouse tumorigenicity test customization, and cell line-derived xenograft model (CDX model) customization.

CELL LINES AND CUSTOMIZATION SERVICES

GemPharmatech has applied its years of CRISPR-Cas9 experience and expertise from generating genetically engineered mouse models to engineering mouse and human cell lines, in particular syngeneic and patient-derived human tumor cells.

Building on top of an extensive collection of 27 syngeneic cell lines, several of the most popular colorectal, breast, melanoma and liver cancer cell lines have been engineered to express human target genes, including popular IO targets such as PDL1, CD47, CD73, CD39, Nectin4, B7H3, ROR1, CD24A, CD39 and more, providing a growing resource for immune checkpoint drug efficacy evaluation as the filed grows and evolves. 21 of such humanized murine cell lines are available for efficacy services provided by GemPharmatech.

To enable in-vivo non-invasive imaging of tumor growth, GemPharmatech has generated 30 luciferase-tagged murine and human cell lines, comprising solid and hematological malignancies. This provides a valuable tool set for evaluating efficacy of new therapies, treating not only solid tumors, but also liquid and metastatic tumors. These cell lines are also available for efficacy services provided by GemPharmatech.

With these successful applications of engineered cell lines in preclinical studies, GemPharmatech offers cell line engineering services to its clients. Ship your wildtype cell lines and we will return custom-engineered cell lines to you.

Syngeneic Cell Lines

GemPharmatech has an extensive collection of syngeneic cell lines, including murine-derived cells and genetic modification of the corresponding cells, as well as several popular target cells, including PDL1, CD47, CD73, CD19, CD20, CD33, EGFR, HER2, BCMA, etc., providing a rich resource of tumor models for immune checkpoint drug efficacy evaluation.

Reporter Gene Cell Lines

GemPharmatech has several Luciferase-modified cell lines, comprising solid and hematological malignancies, which provide a wealth of tumor models for evaluating efficacy of new cancer therapies. What’s special about these lines? Cell lines that are modified to be tagged with Luciferase allows imaging. That means, as cancer cells metastases/develop across the body, it can be imaged because the luciferase-tag makes it glow under a special machine.

CDX Cell Lines

Cell line-derived xenograft models (CDX models) are in vivo models where human-derived tumor cells are transplanted into the subcutis of mice for efficacy evaluation or tumor-related research; It is one of the most frequently used in vivo models for preclinical analyses of anticancer medicines.

GemPharmatech’s extensive selection of CDX cell lines, including pancreatic cancer, breast cancer, colon cancer, lung cancer, and lymphoma, can accommodate the requirements of various pharmacodynamic and efficacy investigations. On top of this wide selection, GemPharmatech has engineered 32 CDX cell lines with luciferase to enable non-invasive tumor volume analysis using bioluminescence.

For more information, please contact us [email protected]