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Struggling to optimize AAV vectors for your gene therapy research? Let us engineer the solution!
In collaboration with PackGene, our AAV Capsid Engineering service is tailored for researchers in South East Asia seeking to outsource high-precision capsid engineering. Together, we deliver cutting-edge solutions that push the boundaries of gene delivery, ensuring unmatched efficiency, specificity, and therapeutic potential.
What We Offer
Our AAV Capsid Engineering service focuses on customizing AAV capsids to improve their tropism and transduction efficiency. By modifying the capsid structure, we can help you achieve superior gene delivery to your desired cell types, ensuring targeted results while minimizing immune responses.
Why Choose Our Service?
Enhanced Tropism
Improved Efficiency
AAV Library Production
Immune Evasion
Tailor AAV capsids for specific cell types using advanced engineering techniques.
Achieve higher transduction rates for more effective gene delivery.
Access our robust AAV Library Service for screening and selecting the ideal capsid variants for your needs.
Optimize capsids to reduce immunogenicity and improve therapeutic outcomes.
What can PackGene’s Ï€-Icosa AAV Capsid Engineering do?
- Boost the precision and efficiency of gene delivery to targeted cell types.
- Reduce off-target effects and lower the risk of triggering unwanted immune responses.
- Develop advanced therapies for cancer and genetic disorders with improved accuracy and effectiveness for treating specific diseases.
Highlights
Never miss the candidate
Combine Directed evolution and rational design
Data you can trust
Consolidated experimental data in animals
Exclusive and confidential
Validated variant sequence will be removed for future projects
Service
Our standard Capsid Engineering service offers comprehensive support, including capsid library design and construction, two rounds of screening, and final validation of the top-performing variant in model animals. Connect with our technical support team to discuss your specific screening criteria, animal testing requirements, and any customized needs.
AAV Capsid Engineering Workflow Details
PackGene’s Ï€-Icosa system for AAV capsid engineering offers a comprehensive AAV service for creating and screening custom AAV libraries to meet specific gene and cell therapy (GCT) needs.
- Phase I: Generate a diverse AAV capsid library through mutation and plasmid production, followed by AAV packaging, animal testing, and NGS analysis. A refined library is designed if needed.
- Phase II: Screen the updated library to identify top-performing variants with improved targeting and specificity.
- Phase III: Produce and validate the best variants through custom AAV production, large-scale animal testing, and optional histology.
This process ensures the identification of optimized AAV capsids for enhanced organ targeting and reduced off-target effects.
Packages
| π-Icosa standard package* | Animal | Injection | Organ |
|---|---|---|---|
| Package 1 | Mouse (Mus musculus) | Intravenous | Any types of Organ except Brain |
| Package 2 | Mouse (Mus musculus) | Intravenous | Brain |
| Package 3 | Cynomolgus monkey (Macaca fascicularis) | Intravenous | Any types of Organ except Brain |
| Package 4 | Cynomolgus monkey (Macaca fascicularis) | Intrathecal | Brain |
*Standard packages include two rounds of screening and final validation.
If you need to customize the project, please contact us.
Excellent pre-screening library diversity and uniformity
A high-coverage AAV library with diverse and distinct capsid variants increases the chances of identifying ideal properties for AAV capsid engineering. Low coverage risks missing critical variants, limiting optimization potential.
Uniformity is equally crucial to ensure equal representation of variants, avoiding screening bias and ensuring optimal results in custom AAV production.
Extremely low mispackaging rate : <0.1%
In AAV capsid engineering, minimizing the mispackaging rate is critical for accurate screening and reliable custom AAV production. High mispackaging rates can lead to incorrect genetic material in AAV vectors, causing unintended transduction, toxicity, or reduced efficacy. Additionally, mispackaging may produce replication-competent AAV (rcAAV), posing safety risks like immune reactions in therapies for cancer or genetic disorders.
To reduce mispackaging, optimizing the vector production process is essential, including precise DNA input control, high-quality plasmid production, and refined transfection conditions. Screening for mispackaging ensures the AAV library remains representative and reliable for testing.
PackGene’s process measures the mispackaging rate by analyzing capsid protein integrity, achieving extremely low rates to match capsid genes with proteins during packaging, ensuring accurate results for AAV capsid engineering.
Performance
Novel Capsid screened from π-Icosa system target to CNS
14 days after lumbar injection in mouse. Sample size=5
AAV-PHP.eB – a well known serotype that specifically targeted to CNS and AAV-PG008, an variant engineering via π-Icosa 10 days after injection.
Novel capsid targeting muscle with liver-detargeting
PG007- Novel capsid targeting muscle
Mouse samples are collected 21 days after virus injection
AAV-PG007, a modified backbone via π-Icosa system based on AAV9 and AAV2 chimera shows a reduction of the off-targeting to the liver while keeping muscle targeting in both mouse and monkey​
PG007 is both efficient and specific in muscle-targeting cross rodent species​
Novel capsid targeting primary human T cells
PG007 delivers micro-dystrophin in DMD mouse models by systematic administration
Muscle function restoration and liver damage recovery​
PG007 shows functional improvement in micro-dystrophin delivery to DMD mouse models by systematic administration​
Micro-dystrophin protein levels in the muscles of via immunofluorescence staining​
PG007 shows functional improvement in micro-dystrophin delivery to DMD mouse models by systematic administration​​
Learn more about our AAV Library Services
in Southeast Asia
Trusted AAV Services Partner in South East Asia
PackGene is the go-to provider for researchers across Southeast Asia, including Singapore, Thailand, Malaysia, and Indonesia. With unmatched expertise and precision, we deliver high-quality services to accelerate your scientific discoveries
Contact us today to find out how our AAV Services can help accelerate your research!
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