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Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (Min X Rat)- Categories:
Antibodies, Conjugates & Labelling, Cell imaging & Analysis, Secondary Antibodies
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Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (Min X Rat)
- Categories:
Antibodies, Conjugates & Labelling, Cell imaging & Analysis, Secondary Antibodies
$0.00
Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit
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Biotium products are distributed only in Singapore and Thailand.
A fixable, fluorescent assay to determine yeast viability. Contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.
Product Description
The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.
Features
- Stable and selective dead cell staining
- Simple, rapid, no-wash staining protocol
- Tolerates formaldehyde fixation
- Suitable for flow cytometry and microscopy
Kit Components
- Live-or-Dye™ 568/583 (solid), 4 vials
- Thiazole Orange 10 mM in DMSO, 100 uL
- Anhydrous DMSO, 250 uL
Spectral Properties
- Live-or-Dye™ 568/583 Ex/Em 568/583 nm
- Thiazole Orange Ex/Em 512/533 nm
How It Works
Live-or-Dye™ 568/583 is a cell membrane impermeable amine-reactive dye. It is able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. This dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. It is suitable for detection by fluorescence microscopy or flow cytometry.
Thiazole Orange is a green fluorescent dye that localizes to the cytoplasm, with some nuclear accumulation. It is fixable, resulting in diffuse cytoplasmic staining in fixed cells.
After staining yeast with this kit, both living and dead cells will be stained with green fluorescence. Only dead cells will be stained with red fluorescence.
Product Attributes
| For live or fixed cells | For live/intact cells |
|---|---|
| Fixation options | Fix after staining (formaldehyde), Permeabilize after staining |
Reference Publications
Arévalo-Jaimes, B.V.; Torrents, E.
Died or Not Dyed: Assessment of Viability and Vitality Dyes on Planktonic Cells and Biofilms from Candida parapsilosis
Journal of Fungi. 2024; 10(3):209.
DOI: https://doi.org/10.3390/jof10030209
Article Snippet: “For the viability assessment, the dyes used were S9 (Live/Dead BacLight Bacterial Viability Kit) (Thermo Fisher Scientific, Carlsbad, CA, USA) at 30 μM and TO (Yeast Live-or-Dye Fixable Live/Dead Staining Kit) (Biotium, Fremont, CA, USA) at 10 μM.”
Le, Phuc H et al.
Surface Architecture Influences the Rigidity of Candida albicans Cells
Nanomaterials (Basel, Switzerland) vol. 12,3 567. 7
DOI: 10.3390/nano12030567
Article Snippet: “ The cells were stained with a Yeast Live-or-Dye™ Fixable Live/Dead staining kit (Biotium, Fremont, CA, USA) that includes Live-or-Dye™, a cell membrane-impermeable red dye with excitation/emission of 562/583 nm that covalently stains free amines on the intracellular proteins of dead cells,”
Phuc H. Le, Denver P. Linklater, et al.
Apoptosis of Multi-Drug Resistant Candida Species on Microstructured Titanium Surfaces
Adv. Mater. Interfaces 2023, 10, 2300314
Article Snippet: “The examined surfaces were carefully washed three times (3 mL each) with MilliQ water to remove non-attached cells and then put in a 3.5 mm glassbottomed Petri dish filled with 3 mL MilliQ water. Yeast Live-or-Dye Fixable Live/Dead staining kit (Biotium, USA) was used to differentiate between the live and dead cells.”
