Selective KBM Human NK Cell Culture Kit – Kohjin Bio
Selective KBM Human NK Cell Culture Kit – Kohjin Bio
$950.00
Boost NK cell production for CAR-NK & NK therapy research! KBM NK Cell Culture Kit delivers high purity (almost 50%) & rapid expansion (100-150x) in 10 days. Scale up your research efficiently.
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Accelerate CAR-NK & NK Cell Manufacturing: High Purity NK Cells in 10 Days (KBM NK Cell Culture Kit)
Accelerate your NK cell research with the KBM Natural Killer Cell Culture Kit. This specialized medium is designed to support the robust selection and expansion of activated human NK cells from human peripheral blood (PBMC), providing a powerful tool for researchers and clinicians in Asia developing cell-based therapies.
Selective KBM NK Cell Culture Kit is an RUO-grade product.
Kohjin Bio’s KBM NK Cell Culture Kit delivers a streamlined solution for generating highly pure NK cells from PBMCs in just 10 days.
This kit tackles your biggest NK cell expansion headaches
- Purity Problems? We’ve got you covered. Achieve almost 50% purity in just 10 days – that’s a massive leap from traditional methods!
- Time Crunch? No sweat. This kit delivers a 100-150-fold increase in NK cells within 10 days. Billions of potent warriors, ready when you are.
- Workflow Woes? We hear you. The pre-coated culture bag and optimized media minimize prep and streamline your process, freeing you to focus on groundbreaking research.
- Built to Scale: Designed for industrial use, this kit easily adapts to your growing research needs. Scale up your NK cell production with ease!
Boost Your Immunology & Immunotherapy Research & Development: The KBM NK Cell Culture Kit Delivers More Than Just Numbers:
- Time & Resource Savings: Efficient NK cell expansion translates to less wasted time and resources. More time for groundbreaking research, and less time for troubleshooting!
- Unparalleled Quality: Obtain the pure NK cell populations critical for successful CAR-NK and NK therapy development.
- Reliable & Reproducible Results: Ensure consistent and dependable NK cell production, keeping your research on track and delivering trustworthy data.
Don’t Let NK Cell Production Hinder Your Breakthroughs!
Order your KBM NK Cell Culture Kit today and experience the power of scalable, high-purity NK cell production. Watch your research take a giant leap forward!
Selective KBM NK Cell Culture Kit is a research-grade product.
P.S. Still have questions about scaling up your NK cell research? Our team of experts at [email protected] is here to help. Let’s conquer the world of NK cell therapy together!
Documents
Safety Data Sheet
KBM NK Kit Basic Protocol/Manual
Please request the KBM NK Kit Protocol from your sales representative/ [email protected] or contact us here.
Sample Culture Protocol
Example of Culture Protocol
1. PBMC 2.0E+07 cells were seeded into T75 flasks pre-coated (NKCC-1 20mL, serum 2mL added) and incubated in a 5% CO2 incubator at 37℃.
2. After 5 days of culture, the cells were collected when the number of cells reached 8.2E+07 cells. Transfer to expansion culture (1200 rpm x 5 min).
3. Add 200 mL of NKCC-2 so that the cell concentration gets 4.0E+05 cells/mL. Add inactivated (56℃, 30 min) human serum (10 mL) obtained at PBMC separation.
4. After 7 days of culture, add the remaining 800nL of NKCC-2 to the bag, after it was confirmed that the medium had become yellowish.
5. After 10 days of culture, count the number of cells, confirm the cell surface markers (CD56, CD3) and measure the cytotoxic activity using K 562 (target cancer cell) on a flow cytometer.
Technical Specifications
KBM NK Kit Technical Specifications:
Components
- KBM NKCC-b (culture bag)
- KBM NKCC-c (coating agent)
- KBM NKCC-1 (primary culture medium)
- KBM NKCC-2 (expansion culture medium)
Preservation
Keep refrigerated (2-8°C) until time of use. (If it isn’t dry, it can be used for several days.)
