Reference Publications

Lu Wang, Tze King Tan, Hyoju Kim, Dennis Kappei, Shi Hao Tan, A. Thomas Look, Takaomi Sanda

ASCL1 characterizes adrenergic neuroblastoma via its pioneer function and cooperation with core regulatory circuit factors

Cell Reports, Volume 42, Issue 12, 2023, 113541, ISSN 2211-1247,

DOI: https://doi.org/10.1016/j.celrep.2023.113541

Article Snippet: “GI-MEN cell lines transduced with the DOX-inducible pCW57.1-ASCL1 vector were cultured in RPMI 1640 medium (Biowest, cat# L0500-500) supplemented with 10% tetracycline-free fetal bovine serum (Biowest, cat# S181T-500) and 1% penicillin/streptomycin (Biowest, cat# L0022-020).”

Radic Shechter, Ksenija et al.

Metabolic memory underlying minimal residual disease in breast cancer

Molecular systems biology vol. 17,10 (2021): e10141.

DOI: doi:10.15252/msb.202010141

Article Snippet: Highlight from the materials and methods table “Fetal Bovine Serum (FBS), Tetracycline Free Biowest S181T“

Marchena-Cruz, Esther et al.

DDX47, MeCP2, and other functionally heterogeneous factors protect cells from harmful R loops

Cell reports vol. 42,3 (2023): 112148.

DOI: doi:10.1016/j.celrep.2023.112148

Article Snippet: “U2OS-TR-AID stable cell line carrying a tetracycline-regulated AID gene cassette was cultured under the same conditions but supplemented with a special Fetal Bovine Serum (FBS) South America, Tetracycline Free (S181T, Biowest). Cells were grown at 37°C and 5% CO₂.”

Turan, Gizem & Olgun, Cagla & Ayten, Hazal & Toker, et al.

Dynamic proximity interaction profiling suggests that YPEL2 is involved in cellular stress surveillance

bioRxiv 2023.07.31.551286.

DOI: 10.1101/2023.07.31.551286. 

Article Snippet: “COS7 cells were maintained and transfected in the DMEM medium containing 10% tetracycline-free FBS (Tet-free FBS, Biowest, France, S181T) to minimize the effect of endogenous tetracycline on transgene expression.”

Hannah, V., Dieudonné, B. K., Valerio, T. et al. Novel non-HAP class A HBV capsid assembly modulators have distinct in vitro and in vivo profiles. ASM Journals. 2023. Doi: https://doi.org/10.1128/jvi.00722-23

Article Snipped: “Cells were then centrifuged; the supernatant was discarded; and the cell pellet was resuspended in high glucose DMEM with 2% Tet-free FBS (Biowest S181T) and 2 mM L-glutamine.”

Dwivedi, D., Harry, D. & Meraldi, P. Mild replication stress causes premature centriole disengagement via a sub-critical Plk1 activity under the control of ATR-Chk1. Nat Commun 14, 6088 (2023). https://doi.org/10.1038/s41467-023-41753-1  

Article Snipped: “…supplemented with 10% tetracycline free fetal calf serum (FCS) (Biowest: S181T-500) and 100 U/ml of each penicillin and streptomycin (P/S) (Thermo Fisher Scientific: 15140122) at 37 °C, 95% relative humidity and 5% CO2 in humidified CO2 incubator”

Ksenija, R. S., Eleni, K., Katharina, Z. et al. Metabolic memory underlying minimal residual disease in breast cancer. Molecular Systems Biology. (2021) 17: e10141. Doi: https://doi.org/10.15252/msb.202010141 

Article Snipped: “STOP media: 500 ml of Dulbecco’s modified Eagle medium:F12, DMEM:F12 with HEPES (15 mM), 1:1 mixture with 3.151 g/l glucose, with l‐glutamine (Lonza BE12‐719F), supplemented with HEPES to the final concentration of 25 mM, 5 ml of penicillin/streptomycin (Gibco, 15140‐122) and 50 ml of fetal bovine serum, tetracycline free (Biowest, S181T).”

Gentian, M., Swati, S., Jana, P. et al. miR-27a/b is a posttranscriptional regulator of Gpr126 (Adgrg6). Annals of the New York Academy of Sciences. 2019. Doi: https://doi.org/10.1111/nyas.14245 

Article Snipped: “HEK293T and HeLa cells were cultured in DMEM+GlutaMAX medium (Gibco, #31331-093) supplemented with 10% fetal bovine serum (Biowest, #S181T) at 37 °C and 5% CO2.”

Tamara, C., Yufeng, Z., Thomas, F. et al. An inhibitor-mediated beta-cell dedifferentiation model reveals distinct roles for FoxO1 in glucagon repression and insulin maturation. Molecular Metabolism. 2021; 54. Doi: https://doi.org/10.1016/j.molmet.2021.101329 

Article Snipped: “Min6 cells were grown in high-glucose DMEM supplemented with 15% Tet System Approved FBS (Biowest S181T), 71 μM 2-mercaptoethanol, 50 U/mL penicillin, and 50 μg/mL streptomycin.”