References

Beiyuan H., Tiantian Z., Wei Q. et al. Inhibition of EGFR Overcomes Acquired Lenvatinib Resistance Driven by STAT3–ABCB1 Signaling in Hepatocellular Carcinoma. Cancer Research. 2022; 82 (20): 3845–3857. Doi: https://doi.org/10.1158/0008-5472.CAN-21-4140 

Article Snipped:  “Cells were cultured in DMEM (Gibco) with 10% lipid depleted FBS (S181L, Biowest) for 24 hours”

Saliakoura, M., Sebastiano, M.R., Nikdima, I. et al. Restriction of extracellular lipids renders pancreatic cancer dependent on autophagy. J Exp Clin Cancer Res 41, 16 (2022). https://doi.org/10.1186/s13046-021-02231-y 

Article Snipped: “AsPC-1 and SU68.86 were cultured in RPMI-1640 (Gibco), MiaPaCa-2, Hs766S, CFPAC-1 and PANC-1 cell lines were cultured in DMEM (Gibco); media were supplied with 10% FBS (Thermo Fisher) or delipidated FBS (via fumed silica precipitation method; Biowest #S181L)”

Elisabetta, V., Giovanni, L. B., Mariachiara, A. et al. Targeting of the lipid metabolism impairs resistance to BRAF kinase inhibitor in melanoma. Frontiers in Cell and Developmental Biology. 2022; 10. Doi; https://doi.org/10.3389/fcell.2022.927118 

Article Snipped: “ Lipid starvation was carried out replacing FBS with lipid-depleted serum (S181L, Biowest, Voden Medical Instruments, Meda, Italy).”

Zhiwen, F., Ming, K., Min, L. et al. Brahma related gene 1 (Brg1) regulates cellular cholesterol synthesis by acting as a co-factor for SREBP2. Frontiers in Cell and Developmental Biology. 2020; 8. Doi: https://doi.org/10.3389/fcell.2020.00259 

Article Snipped: “To induce SREBP2 activity, cells were cultured in the LDM1 media containing lipid-depleted fetal bovine serum (Biowest, cat# S181L).”

Marianne, R., Claire, H., Rebecca, M. et al. Trafficking of cholesterol to the ER is required for NLRP3 inflammasome activation. Journal of Cell Biology. (2018) 217 (10): 3560–3576. Doi: https://doi.org/10.1083/jcb.201709057 

Article Snipped: “BMDMs were seeded into 24- or 12-well plates and incubated overnight in media containing 5% lipid-depleted serum (S181L; Biowest)”