References

Yin-Yan H, Bin C, Yi-Xia Y, et al. Estrogenic G protein-coupled receptor 30 signaling is involved in regulation of endometrial carcinoma by promoting proliferation, invasion potential, and interleukin-6 secretion via the MEK/ERK mitogen-activated protein kinase pathway. Cancer science. 2009; 100(6). Doi;  https://doi.org/10.1111/j.1349-7006.2009.01148.x 

Article Snipped: “The cells were further cultured for 48 h with phenol red-free DMEM/F12 containing 5% dextran-coated charcoal-treated FBS (S181F; Biowest).”

Ping J, Jinwen X, Shuhui Z, et al. 17β-estradiol down-regulates lipopolysaccharide-induced MCP-1 production and cell migration in vascular smooth muscle cells. Journal of Molecular Endocrinology. 2010; 45(2). Doi: https://doi.org/10.1677/JME-09-0166 

Article Snipped: “Cells were grown in a 5% CO2 atmosphere at 37 °C in DMEM without phenol, and supplemented with penicillin and streptomycin, and 10% fetal bovine serum charcoal was stripped (steroid free and delipidated, FCS; Biowest, S181F-500, Nuaillé, France).”

Shuhui Z, Peng S, Haimei L, et al. 17β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells. PLOS ONE. 2018. Doi: https://doi.org/10.1371/journal.pone.0202500 

Article Snipped: “Cells were grown in a 5% CO2 atmosphere at 37°C in DMEM without phenol, supplemented with penicillin and streptomycin, and 10% charcoal-stripped FBS (steroid free and delipidated, fetal bovine serum) (Biowest, S181F-500, Nuaille, France).”

Jia, L., Kevin, A. L., Frans, E. T., John, C. W. BRS1, a serine carboxypeptidase, regulates BRI1 signaling in Arabidopsis thaliana. PNAS. 2001; 98 (10) 5916-5921. Doi: https://doi.org/10.1073/pnas.091065998 

Article Snipped: “S181F and H438A mutants were introduced with a QuickChange Site-Directed Mutagenesis Kit (Stratagene) in a pBluscript SK+ vector.”