Phosphatase Inhibitor Cocktail I (100× ddH2O)
Phosphatase Inhibitor Cocktail I (100× ddH2O)
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It is easy for the proteins to be degraded or dephosphorylated during in vitro extraction procedure, which could cause an inaccurate result of protein expression detection. Therefore, adding protease inhibitors or phosphatase inhibitors into the extracts would be an effective method to prevent degradation and dephosphorylation of protein.
The phosphatase inhibitor mixture can effectively inhibit the dephosphorylation of common phosphatases on proteins and maintain the original phosphorylation state of proteins.
All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Composition
| Ingredient | CAS | MW | Concentration (100x) | Target | Type |
|---|---|---|---|---|---|
| Sodium Fluoride | 7681-49-4 | 41.99 | 100 mM | Acid Phosphatase | Reversible |
| Sodium Orthovanadate | 13721-39-6 | 183.91 | 100 mM | Alkaline phosphatase, PTPs, ATPases | Reversible |
| Sodium Molybdate | 7631-95-0 | 205.92 | 115 mM | Acid and phosphoprotein Phosphatase | Irreversible |
| Sodium Tartrate | 868-18-8 | 194.05 | 400mM | Acid Phosphatase | Reversible |
| Imidazole | 288-32-4 | 68.08 | 200 mM | Alkaline phosphatase | Reversible |
Features
- Accurate Ingredients —— Clearance of components and concentrations to avoid experimental uncertainty
- Comprehensive Protection—— Effectively inhibit various proteases to protect proteins from being degraded
- Cost-Effective —— Lower price with higher quality
Handling Instruction
- Compatible with Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, kinase assay and etc.
- Add concentrated cocktail at 1:100 (v/v) dilution to samples solution (such as cell lysates or tissue extracts) before assaying.
- Briefly vortex cocktails to help facilitate the dissolution.
Notice
- Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
- If Cocktail I and Cocktail II are used together, do not mix tube I with tube II beforehand, as there may be precipitation. To avoid this, please add them step by step during experiment.
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