Iscove’s Modified Dulbecco’s Medium (IMDM) is a versatile cell culture medium specifically designed to support a wide range of cell types. As a modification of DMEM, IMDM contains additional nutrients, including selenium, amino acids, vitamins, sodium pyruvate, and HEPES buffer.
Key Features:
- Enhanced nutrient composition: Provides essential nutrients for optimal cell growth and function.
- Versatility: Supports a variety of cell types, including murine B lymphocytes, hemopoietic tissue, and hybrid cells.
- Buffered pH: HEPES buffer maintains stable pH for optimal cell culture conditions.
- Phenol red-free: Minimizes interference with light-sensitive assays.
- Stable glutamine: Ensures long-term stability and eliminates the need for supplemental glutamine.
Recommended Applications:
- Primary cell culture: Ideal for cultivating bone marrow-derived cells, B cells, and T lymphocytes.
- Hybridoma culture: Supports the growth and production of monoclonal antibodies.
- Cell line maintenance: Provides a suitable environment for various cell lines.
Storage and Handling:
- Store at 2-8°C, protected from light and humidity.
- Avoid repeated freeze-thaw cycles.
- Handle under aseptic conditions to prevent contamination.
- Supplement with antibiotics as needed.
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