OMEGA96
Neuronal Co-Culture Microplate

1 x OMEGA⁹⁶ screening plate, 48 experiments! Equivalent to 24 OMEGA⁴ or 48 devices from competitors, at a fraction of the price!

The first of its kind. It is the scaled up version of the OMEGA⁴, High-Throughput Screening (HTS) Compatible.

Speed and scalability are the key to rapidly identifying new therapeutics. Owing to the unique open-top chamber design, the OMEGA⁹⁶ plate is the ONLY microfluidic platform commercially available that fully conforms to the 96-well microplate standard. As such, the OMEGA⁹⁶ plate is compatible with most lab automation and robotic systems such as microplate washers, plate readers, high-content imagers, pinning tools and plate hotels.

The OMEGA⁹⁶ screening plate opens up the way to so many assays. In a basic application, the plate serves as a compartmentalization tool to isolate the somatic and axonal components of seeded neurons. This clarifies the results from toxic species uptake assays and makes glutamate toxicity assays a breeze to setup. Used as a co-culture device,  the OMEGA⁹⁶ is an ideal platform to study neurodegeneration, the neuromuscular junction (NMJ), the nigrostriatal pathway, pain pathways, myelination, as well as chemotactic-releasing cultures.

OMEGA⁹⁶ plate features:

• Ready-to-use: sterile and pre-bonded to a high-transmissive glass coverslip
• Optionally  pre-coated with either poly-D-lysine or poly-L-ornithine
  
• 48 experiments can be performed per plate – perfect for screening!
• Open-top chamber design provides full access to your cultures – use explants or spheroids in your experiments

Just starting out? Then you might want to try our OMEGA⁴ device first.

Need a third culture chamber? The OMEGA^96-ACE might be what you are looking for!

Description

High-Throughput Screening Compatible

The OMEGA series of microfluidic devices from eNUVIO are perfectly suited for experiments involving neuronal co-culture and compartmentalization. Each of the chambers of the device are physically separated by a series of microfluidic channels. The small size of the microchannels serve to fluidically isolate each of the adjoined chambers, but are large enough to allow neurites/axons to project into and interact with cultures located in the adjacent chamber.

The unique open-chamber design of the OMEGA gives scientists direct access to their co-cultures, enabling researchers to explore new types of experimental paradigms not possible with standard closed-chamber devices.  For example, the chambers are large enough to house tissues that are several millimeters in diameter, easily accommodating tissue explants or self-assembled 3D cultures (e.g. spheroids, organoids).

OMEGA⁹⁶ plates are shipped ready to use. They are sterile, pre-bonded to high-transmissive glass, and optionally pre-coated with either poly-D-lysine or poly-L-ornithine. Maintaining healthy cultures over a long period of time is even easier with OMEGA plates, owing to the evaporation-minimizing tracks around the edge of each plate. 

OMEGA⁹⁶ devices are suited for in vitro co-culture studies involving:

• Neuromuscular junction (NMJ) in the context of ALS, Kennedy’s disease (SBMA), Duchenne’s muscular dystrophy
  • e.g. motor neuron/muscle co-culture

• Nigrostriatal pathway in the context of Parkinson’s disease
  • e.g. dopaminergic neuron (SNc)/GABAergic neuron (striatal) co-culture

• Sensory system in the context of nociception and mechanosensation
  • e.g. skin fibroblast/DRG co-culture

• Myelination
  • e.g. neuron/oligodendrocyte or Schwann cell co-culture

• Enteric nervous system
  • e.g. enteroendocrine cells/vagal neuron co-culture

Culture compatibility:

• iPSC-derived cultures
• Self-assembled 3D cultures (e.g. spheroids, organoids)
• Primary cultures
• Tissue explants
• Immortalized Cell lines

OMEGA⁹⁶ specifications:

• 48 well-pairs per microplate = 48 individual experiments per plate
• LxWxH: 127.2 x 85.3 x 14.1 mm
• Chamber surface area: ~0.35 cm²
• Chamber diameter: 6 mm
• Chamber volume: 350 μL
• Chamber pitch: 9 mm
• Microchannel width: 10 μm
• Microchannel length: ~640 μm
• Microchannels/chamber pair: 70
• Pre-bonded glass bottom: 200 μm thick
• Shipped sterile in PBS, ready to use
• Hydrophilic, no need for plasma treatment
• Option: pre-coated with poly-D-lysine or poly-L-ornithine
• Anti-evaporation tracks around the edge of each plate

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