OMEGA ACE
Triple-Chamber Neuronal Co-Culture

Let your neurons do what they were meant to do – make synaptic connections! Get even more out of your neuronal cultures and co-cultures with the latest in microfluidic devices from eNUVIO.

OMEGA^ACE devices can be used for in vitro triple co-culture studies of neurodegeneration, neuromuscular junction (NMJ), nigrostriatal pathway, pain, myelination, or chemotactic factors. In single culture applications, the OMEGA^ACE can also serve as a compartmentalization tool to separate the somatic, proximal axonal and distal axonal (growth cone) components of neurons.

OMEGA^ACE device features:

• Ready-to-use: sterile, hydrophilic, and pre-bonded to a high-transmissive glass coverslip
• Open-top chamber design provides full access to your cultures – use explants or spheroids in your experiments!
• Choose between Starter Kit and Refill Kit

Do you need a wider middle chamber? Then the OMEGA^ACE-2mm or the HTS compatible OMEGA^96-ACE microplate might be right for you.

Description

The OMEGA series of microfluidic devices from eNUVIO are perfectly suited for experiments involving neuronal co-culture and compartmentalization. Each of the growth chambers of the device are physically separated by a series of microfluidic channels that serve to fluidically isolate each of the adjoined chambers. These microchannels are large enough however, to allow for neurites/axons to project into and interact with cultures located in the adjacent chamber. OMEGA^ACE devices each contain three serially-adjoined culture chambers. Compared to our two-chamber devices, triple-chamber devices provide researchers with the ability to perform more specialized co-culture and compartmentalization experiments.

The unique open-chamber design of OMEGA series devices gives scientists direct access to their cultures, enabling researchers to explore new types of experimental paradigms not possible with standard closed-chamber devices.  For example, the chambers are large enough to house tissues that are several millimeters in diameter, accommodating tissue explants or self-assembled 3D cultures (e.g. spheroids, organoids). Additionally, conventional patch-clamp electrodes can openly access the cultures, allowing researchers to examine the electrophysiological properties of the culture.

OMEGA devices are shipped out ready to use. They are sterile, hydrophilic and are pre-bonded to high-transmissive #1.5 glass coverslips. Simply coat the growth chambers and plate your cells – it’s that easy. Maintaining healthy cultures over a long period of time is even easier with OMEGA devices, owing to the evaporation-minimizing inserts included in every kit. Not only do these simple reusable culture inserts contain a built-in circular well that reduces the evaporation rate from the device’s chambers when filled with fluid, they also serve to physically stabilize the device for secure transportation of the device within the culture room.

OMEGA^ACE devices can be used for in vitro triple co-culture, myelination, and triple compartmentalization studies. For example, motor neuron, Schwann cell, skeletal muscle  can be co-cultured in the context of neurodegeneration (ALS, Kennedy’s disease (SBMA), Duchenne’s muscular dystrophy). The third chamber can also serve to separate the somatic, proximal axonal, and distal axonal (growth cone) components of neurons for specialized uptake experiments of potentially toxic protein species (e.g. α-synuclein PFF, Tau) or compartment-specific application of pharmacologically active molecules.

Culture compatibility:

• iPSC-derived cultures
• Self-assembled 3D cultures (e.g. spheroids, organoids)
• Primary cultures
• Explant tissues
• Immortalized cell lines

OMEGA^ACE specifications:

• Glass coverslip: 22 mm, round, #1.5 thickness
• Chamber #2 channel width: 0.25 mm
• Chamber #2 channel height: 0.5 mm
  
• Number of microchannels per interface: 70
• Number of interfaces per device: 2
• Microchannel length: >630 µm

The OMEGA^ACE Starter Kit includes:

• 4 x OMEGA^ACE devices
• 4 x evaporation minimizing culture inserts (for 35 mm dishes)
• 4 x sterile culture dishes (35 mm, round)
• 1 x microscopy adapter for OMEGA series devices

The OMEGA^ACE Refill Kit includes:

• 4 x OMEGA^ACE devices
• 4 x sterile culture dishes (35 mm, round)

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