Reference Publications

Amit J. Sabnis; Steven Chen; Carolina Morales; Benjamin S. Braun

Abstract A27: An apoptosis-based screen for targeted agents in rhabdomyosarcoma reveals potential combination therapies

Cancer Res 15 July 2020; 80 (14_Supplement): A27.

DOI: https://doi.org/10.1158/1538-7445.PEDCA19-A27

Article Snippet: “We built a screening assay using high-throughput microscopy to quantify both cell number (Hoechst nuclear staining) and apoptosis (NucView 488 Caspase-3 Enzyme Substrate [Biotium]). Technical controls included DMSO and staurosporine.”

Jordan F Hastings, Alvaro Gonzalez Rajal, et al.

Analysis of pulsed cisplatin signalling dynamics identifies effectors of resistance in lung adenocarcinoma

eLife 9:e53367.

DOI: https://doi.org/10.7554/eLife.53367

Article Snippet: Highlight from the figures and data table “Commercial assay or kit NucView 488 Caspase-3 Enzyme substrate Biotium #10402″

Florian Guisier, Pierre Bohn, et al.

In- and ex-vivo molecular imaging of apoptosis to assess sensitivity of non-small cell lung cancer to EGFR inhibitors using probe-based confocal laser endomicroscopy

PLoS ONE 12(7): e0180576.

DOI: https://doi.org/10.1371/journal.pone.0180576

Article Snippet: “The NucView 488 Caspase-3 substrate (Biotium, San Francisco, California, USA), hereafter referred to as C3-NucView, is a fluorogenic dye that detects caspase 3 activity within intact cells, without interfering with the caspase 3 activity.“

Masahiro YAMAMURA, Akira YAMAUCHI, Shigenobu TONE, Yoshiyuki YAMAGUCHI

Sorafenib-induced apoptosis in colonic neuroendocrine carcinoma cells

Kawasaki Medical Journal 48：175－182，

DOI: 10.11482/KMJ-E202248175

Article Snippet: “Caspase-3 activation was analysed using the NucView 488 Caspase-3 Assay Kit for live cells (Biotium, San Francisco, CA, USA), a caspase-3 substrate that fluoresces when cleaved.”

Vasconcelos et al.

Individual Human Cytotoxic T Lymphocytes Exhibit Intraclonal Heterogeneity during Sustained Killing

Cell Reports 11, 1474–1485

DOI: http://dx.doi.org/10.1016/j.celrep.2015.05.002

Article Snippet: “For live cell imaging, 1 3 105 pulsed target cells were seeded into microchambers (Lab-Tek Chambered coverglass; Nalgen Nunc) previously coated with poly-D lysine, followed by addition of 2 mM NucView 488 caspase-3 substrate (Biotium)”

Maurice Bosch and Vernonica E. Franklin-Tong

Temporal and spatial activation of caspase-like enzymes induced by self-incompatibility in Papaver pollen

PNAS 104 (46) 18327-18332

DOI: https://doi.org/10.1073/pnas.0705826104

Article Snippet: “The NucView 488 caspase-3 assay Kit (Biotium, Inc., Hayward, CA) was used to detect DEVDase activity in live pollen tubes. The specificity of the DEVDase activity was checked by pretreating hydrated pollen for 1 h with 100 μM Ac-DEVD-CHO or Ac-YVAD-CHO before SI induction.”