Biotium products are distributed only in Singapore and Thailand.
These kits allow optimal labeling of single-chain nanobodies with CF® dyes or biotin in 30 minutes without a purification step. The labeling tolerates common buffer components including BSA.
Product Description
Mix-n-Stain™ Nanobody Labeling Kits are designed specifically for labeling single-domain Nanobodies® (also called camelid single variable or VHH domains) with one of Biotium’s bright and photostable CF® dyes or biotin. The kits allow optimal labeling of between 5-50 ug of nanobody in just 30 minutes, with minimal hands-on time and no purification. Labeling tolerates common buffer additives, including BSA.
The kits come with a selection of our next-generation CF® dyes or biotin. CF® dyes have advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes (learn more about CF® Dyes).
Features
- Label 5-20 ug or 20-50 ug of Nanobody®
- Choice of 7 CF® Dye colors or biotin
- Simple 30 minute labeling, no purification
- Compatible with common stabilizers
- Dye options for super-resolution and 2-photon imaging (learn more)
Choosing the Right Kit for Your Application
Before selecting a Mix-n-Stain™ Nanobody Labeling Kit, please download the Product Protocol to see our Kit Compatibility and Protocol Selection guidelines.
For labeling IgG antibodies, please use one of our Mix-n-Stain™ Antibody Labeling Kits. We also offer Mix-n-Stain™ Small Ligand Labeling Kits, for labeling SNAP-tag®, CLIP-tag™, and HaloTag® ligands, or other small molecules. See Mix-n-Stain™ Product Lines below for our full selection of antibody, protein, and ligand labeling kits.
Mix-n-Stain™ Nanobody Labeling Kits
Label | Ex/Em | Labeling size (ug nanobody) |
Catalog number |
---|---|---|---|
Biotin | N/A | 5-20 ug | 92500 |
20-50 ug | 92501 | ||
CF®405S | 404/431 nm | 5-20 ug | 92502 |
20-50 ug | 92503 | ||
CF®488A | 490/515 nm | 5-20 ug | 92504 |
20-50 ug | 92505 | ||
CF®568 | 562/583 nm | 5-20 ug | 92506 |
20-50 ug | 92507 | ||
CF®594 | 593/614 nm | 5-20 ug | 92508 |
20-50 ug | 92509 | ||
CF®640R | 642/662 nm | 5-20 ug | 92510 |
20-50 ug | 92511 | ||
CF®647 | 650/665 nm | 5-20 ug | 92512 |
20-50 ug | 92513 | ||
CF®680R | 680/701 nm | 5-20 ug | 92514 |
20-50 ug | 92515 |
Note: Before selecting a kit, please download the Product Protocol to see Kit Compatibility and Protocol Selection guidelines.
Nanobody is a registered trademark of Ablynx, a Sanofi company. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific. Cy Dye is a registered trademark of GE Healthcare. SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation.