Reference Publications

Brodaczewska, K.K., Bielecka, Z.F., Maliszewska‑Olejniczak, K., Szczylik, C., Porta, C., Bartnik, E., & Czarnecka, A.M.

Metastatic renal cell carcinoma cells growing in 3D on poly‑D‑lysine or laminin present a stem‑like phenotype and drug resistance.

Oncology Reports, 42, 1878-1892. (2019).

DOI: https://doi.org/10.3892/or.2019.7321

Article Snippet: “…and then detached with Accutase (cat. no. L0950; Biowest) to obtain single-cell suspensions, washed 2 times with serum-free and antibiotic-free DMEM (product code, L0501; Biowest) to remove remaining FBS and used in experiments.”

Paolo Manzini, Valeria Peli, Araceli Rivera-Ordaz, Silvia Budelli, Mario Barilani, Lorenza Lazzari

Validation of an automated cell counting method for cGMP manufacturing of human induced pluripotent stem cells

Biotechnology Reports, Volume 33 ,2022, e00708, ISSN 2215-017X,
DOI: https://doi.org/10.1016/j.btre.2022.e00708.

Article Snippet: “To obtain single cell suspensions, hiPSC were incubated with accutase (L0950; Biowest, Nuaillé, France) for 5 min at 37 °C and collected in d-PBS without Ca²⁺ and Mg²⁺ (ECB4004L; Euroclone, Pero, Italy).”

Ramirez Ortega, D., Ovalle Rodríguez, P., Pineda, B. et al.

Kynurenine Pathway as a New Target of Cognitive Impairment Induced by Lead Toxicity During the Lactation. 

Sci Rep 10, 3184 (2020).

DOI: https://doi.org/10.1038/s41598-020-60159-3

Article Snippet: “Mice brains were washed with cold PBS 1Χ and cut into small pieces, then 1 ml of accutase (cat. No. L0950, BioWest) was added.”

Cuenca-Ortolá I, Martínez-Rojas B, Moreno-Manzano V, García Castelló M, Monleón Pradas M, Martínez-Ramos C, Más Estellés J.

A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes.

Biomedicines. 2022; 10(11):2736.

DOI: https://doi.org/10.3390/biomedicines10112736

Article Snippet: “Neurospheres were dissociated before seeding using Accutase (L0950-100, Biowest) following the manufacturer’s instructions. Dissociated NPCs were seeded in 2 drops (6 × 10⁵ cells/3 µL drop) to promote their dispersion around the PLA membrane.”

Reeh, H., Rudolph, N., Billing, U. et al.

Response to IL-6 trans- and IL-6 classic signalling is determined by the ratio of the IL-6 receptor α to gp130 expression: fusing experimental insights and dynamic modelling.

Cell Commun Signal 17, 46 (2019)

DOI: https://doi.org/10.1186/s12964-019-0356-0

Article Snippet: “After stimulation with IL-6 or Hy-IL-6 as indicated, HepG2 and HepG2-IL-6Rα cells were detached from cell culture dishes with 1 ml Accutase (Biowest, Nuaillé, France, Cat. No. L0950–100).”

Maxime RF Gosselin, Virginie Mournetas, Malgorzata Borczyk, Suraj Verma, Annalisa Occhipinti, Justyna Róg, Lukasz Bozycki, Michal Korostynski, Samuel C Robson, Claudio Angione, Christian Pinset, Dariusz C Gorecki

Article Snippet: “Cells were detached from dishes using Accutase (Biowest L0950). “

Article Snippet: “HiPSCs were dissociated with Accutase (Biowest, L0950) into a single cell suspension.”