Dulbecco’s Phosphate Buffered Saline w/o Calcium w/o Magnesium

Dulbecco’s Phosphate Buffered Saline w/o Calcium w/o Magnesium

Dulbecco’s Phosphate Buffered Saline w/o Calcium w/o Magnesium

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Preserve Cell Integrity with Biowest PBS

Biowest offers premium phosphate buffered saline (PBS) solutions, specifically designed to maintain cell integrity and support various laboratory procedures. Our PBS products are rigorously tested and quality-controlled to ensure optimal performance.

Key Benefits:

  • Sterility: Ensures a contamination-free environment for your cell cultures.
  • Physiologically balanced: Provides a suitable environment for a wide range of mammalian cells.
  • Calcium and magnesium-free: Compatible with trypsin-based dissociation methods.
  • Versatility: Ideal for cell enumeration, washing, resuspension, and chromatography.
  • Long shelf life: Maintain product integrity for up to 48 months.

 

Trust Biowest for your cell culture essentials.

We deliver Biowest solutions across South East Asia (Singapore, Thailand, Vietnam, Indonesia, Philippines, Myanmar) and Across China Only.

Reference Publications

Mazurek S, Oleksiewicz U, Czerwińska P, Wróblewska J, Klimczak M, Wiznerowicz M.

Disruption of RING and PHD Domains of TRIM28 Evokes Differentiation in Human iPSCs

Cells. 2021; 10(8):1933.

DOI: https://doi.org/10.3390/cells10081933

Article Snippet: After incubation, the enzyme was removed, the cells were gently washed with DPBS (#L0615, Biowest, Nuaillé, France), and a fresh iPSC medium was added.”

 

Olena Karatsai, Lilya Lehka, Dominika Wojton, Anna Izabela Grabowska, Monika Katarzyna Duda, Robert Lenartowski, Maria Jolanta Redowicz

Unconventional myosin VI in the heart: Involvement in cardiac dysfunction progressing with age

Biochimica et Biophysica Acta (BBA) – Molecular Basis of Disease
Volume 1869, Issue 6,2023,166748,ISSN 0925-4439
DOI: https://doi.org/10.1016/j.bbadis.2023.166748

Article Snippet: “Mice and isolated hearts (without atria and aorta) of the examined ages (N ≥ 11 for E14.5, N ≥ 18 for P0, N ≥ 16 for 3m, and N ≥ 14 for 12m) were weighed. Dissected hearts were palpitated in ice-cold phosphate buffered saline (PBS; L0615–500, Biowest, France) using curved shank forceps to remove the residual blood inside the heart.”

 

Kulig K, Bednaruk K, Rudolphi-Szydło E, Barbasz A, Wronowska E, Barczyk-Woznicka O, Karnas E, Pyza E, Zuba-Surma E, Rapala-Kozik M, et al.

Stress Conditions Affect the Immunomodulatory Potential of Candida albicans Extracellular Vesicles and Their Impact on Cytokine Release by THP-1 Human Macrophages

International Journal of Molecular Sciences. 2023; 24(24):17179.

DOI: https://doi.org/10.3390/ijms242417179

Article Snippet: “Briefly, yeast cells were scratched from agar plates and transferred to the Eppendorf tube with 1 mL of PBS, pH 7.4 (Cat # L0615; Biowest, Nuaillé, France), and gently stirred with the sterile loop.”

 

Da Silva, A. J., Hästbacka, H. S. E., Puustinen, M. C., Pessa, J. C., Goult, B. T., Jacquemet, G., Henriksson, E., & Sistonen, L.
A subpopulation of Talin 1 resides in the nucleus and regulates gene expression
bioRxiv (Cold Spring Harbor Laboratory). (2022).

Article Snippet: “Cells were washed and collected in PBS (L0615, BioWest).”

 

Luoto, J. C., Coelho‐Rato, L. S., Jungarå, C., Bengs, S. H., Roininen, J., Eriksson, J. E., Sistonen, L., & Henriksson, E.
Cancer cell invasion alters the protein profile of extracellular vesicles
Journal of Extracellular Biology2(12) (2023).

Article Snippet: The clear supernatant was collected and the remaining cell-Matrigel pellet was dissolved in 14 mL of phosphate-buffered saline (PBS, L0615, Biowest) and centrifuged at 300 × g for 10 min.”

 

Karoglu-Eravsar, E. T., Tuz-Sasik, M. U., Karaduman, A., Keskus, A. G., Arslan-Ergul, A., Konu, O., Kafaligonul, H., & Adams, M. M.
Long-Term Acetylcholinesterase Depletion Alters the Levels of Key Synaptic Proteins while Maintaining Neuronal Markers in the Aging Zebrafish (Danio rerio) Brain
Brain. Gerontology69(12), 1424–1436. (2023).

Article Snippet: Frozen brain tissues from another cohort were placed in 150 μL of phosphate-buffered saline (L0615; Biowest, Nuaillé, France) and homogenized by passing through a 1 mL (26 gauge) syringe. “

 

 

Lismont, C., Revenco, I., Li, H., Costa, C. F., Lenaerts, L., Hussein, M. a. F., De Bie, J., Knoops, B., Van Veldhoven, P. P., Derua, R., & Fransen, M.

Peroxisome-Derived hydrogen peroxide modulates the sulfenylation profiles of key redox signaling proteins in FLP-In T-REx 293 cells

Frontiers in Cell and Developmental Biology, 10. (2022).

DOI: https://doi.org/10.3389/fcell.2022.888873

Article Snippet: “Cells were grown to 60–80% confluency, trypsinized, collected in cell culture medium, pelleted (150 x g, 5 min), and washed once with DPBS without calcium and magnesium (BioWest, L0615).

 

Dreisig, K., Sund, L., Dommer, M. W., Kristensen, N. P., Boddum, K., Viste, R., Fredholm, S., Odum, N., Jäättelä, M., Skov, S., & Kornum, B. R.
Human P2Y11 expression level affects human P2X7 Receptor-Mediated Cell death.
Frontiers in Immunology9. (2018).

Article Snippet: 106 cells were placed per tube and washed with phosphate buffered saline (PBS) (#L0615-500, Biowest), 400 g for 5 min at 4°C before staining with Fixable Viability Dye eFlouro 506 (#65-0866, eBioscience) for 20 min at RT.”

 

Appenroth, D., Ravuri, C. S., Torppa, S. K., Wood, S. H., Hazlerigg, D. G., & West, A. C.

The reindeer circadian clock is rhythmic and temperature-compensated but shows evidence of weak coupling between the secondary and core molecular clock loops

bioRxiv (Cold Spring Harbor Laboratory). (2024).

DOI: https://doi.org/10.1101/2024.04.24.590752

Article Snippet: “Digested fragments were transferred into a 50 ml falcon tube filled with warm phosphate-buffered saline (PBS, Biowest L0615) and centrifuged for 5 min at 200 x g.”

 

Luoto, J. C., Coelho-Rato, L. S., Bengs, S. H., Roininen, J., Eriksson, J. E., Sistonen, L., & Henriksson, E.
In vivo-mimicking 3D cultures secrete distinct extracellular vesicles upon cancer cell invasion
bioRxiv (Cold Spring Harbor Laboratory) (2020).

Article Snippet: “The clear supernatant was collected and the remaining cell-Matrigel pellet was dissolved in 14 ml of phosphate-buffered saline (PBS, L0615, Biowest) and centrifuged at 300 g for 10 min.”

Documents

MSDS

Technical Data Sheet

Composition