Iscove’s Modified Dulbecco’s Medium (IMDM) is a versatile cell culture medium specifically designed to support a wide range of cell types. As a modification of DMEM, IMDM contains additional nutrients, including selenium, amino acids, vitamins, sodium pyruvate, and HEPES buffer.
Key Features:
- Enhanced nutrient composition: Provides essential nutrients for optimal cell growth and function.
- Versatility: Supports a variety of cell types, including murine B lymphocytes, hemopoietic tissue, and hybrid cells.
- Buffered pH: HEPES buffer maintains stable pH for optimal cell culture conditions.
- Stable Glutamine: Prevents intramolecular cyclization, ensuring long-term stability.
- Quality assurance: Manufactured under stringent quality control standards.
Recommended Applications:
- Primary cell culture: Ideal for cultivating bone marrow-derived cells, B cells, and T lymphocytes.
- Hybridoma culture: Supports the growth and production of monoclonal antibodies.
- Cell line maintenance: Provides a suitable environment for various cell lines.
Storage and Handling:
- Store at 2-8°C, protected from light.
- Avoid repeated freeze-thaw cycles.
- Handle under aseptic conditions to prevent contamination.
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Reference Publications
Zheng, Y., Fang, D., Huang, C., Zhao, L., Gan, L., Chen, Y., & Liu, F.
Gentiana scabra Restrains Hepatic Pro-Inflammatory Macrophages to Ameliorate Non-Alcoholic Fatty Liver Disease.
Frontiers in Pharmacology, 12. (2022).
Article Snippet: “Meanwhile, the femur and tibia bones were isolated and cut to obtain marrows, which were filtrated through 70 μm cell strainer (352350, BD Biosciences, United States), incubated with red blood cell lysis buffer (R1010, Solarbio, China), and centrifuged to purification. Finally, the isolated bone marrow cells were cultured with IMDM (L0191, Biowest, France) containing 10 ng/ml M-CSF (ab129146, Abcam, United Kingdom) for the co-culture assay.”