KBM591 is a versatile cell culture medium specifically designed for the activation and expansion of human lymphocytes.

This serum-free, xeno-free formulation provides an optimal environment for T cell and NK cell culture. 

KBM591 is a RUO product.

Key Features:

• Versatility: Supports both T cell and NK cell culture.
• Xeno-free: Does not contain non-human animal components, reducing reliance on autologous plasma and lowering culture costs..
• Serum-free: Offers a defined and controlled culture environment.
• Customizable: Can be supplemented with serum for specific applications.
• Antibiotic-free: Provides flexibility for adding desired antibiotics.

Recommended Applications:

• Lymphocyte research: Studying T cell and NK cell function and activation.
• Immunotherapy: Developing cell-based therapies for various diseases.

Proliferation for T cell

【Cells】 Human peripheral blood

【Medium】KBM 591

【Vessel】 24 well plate coated with anti-CD3

【Protocol】Seeded 4×105 cells/well and incubated statically at 37℃・5% carbon dioxide.

Added KBM591(with IL-2) according to growth, and cultured for 14 days.

Proliferation for NK cell

【Cells】 Human peripheral blood

【Medium】KBM 591

【Vessel】 24 well plate coated with NKCC-c

【Protocol】 Seeded 1×106 cells/well and incubated statically at 37℃・5% carbon dioxide.

Added KBM591(with IL-2) according to growth, and cultured for 14 days.