KBM591 Advanced Serum-Free T Cell Expansion Media

KBM591 Advanced Serum-Free T Cell Expansion Media

KBM591 – Advanced Serum-Free T-Cell Expansion Media

Optimized for High-Yield T Cell Activation & Expansion

KBM591 is a serum-free, xeno-free cell culture medium specifically engineered for high-efficiency T cell expansion, while also supporting NK cell culture. Ideal for immunotherapy development and lymphocyte research, this versatile formulation delivers consistent, high-density T-cell growth without animal-derived components.

KBM591 is a RUO product.

Key Benefits for T-Cell Expansion

✔ Superior T Cell Proliferation – Achieve robust expansion rates for human peripheral blood T cells in controlled conditions.

✔ Serum-Free & Xeno-Free – Eliminates batch variability and reduces reliance on autologous plasma, lowering costs while ensuring reproducibility.

✔ Ready-to-Use or Customizable – Use as a standalone medium or supplement with serum/cytokines (e.g., IL-2) for tailored applications.

✔ Antibiotic-Free Flexibility – Add only the antibiotics or additives your protocol requires.

✔ Regulatory-Friendly – RUO product with a defined formulation, reducing risks for preclinical workflows.

✔ Validated Performance – Proven in 14-day T cell expansion protocols (see data below).

 

Recommended Applications:

  • CAR-T/TCR-T Therapy Development – Scale T cell production for immunotherapy pipelines.
  • T Cell Research – Study activation, exhaustion, or tumor-infiltrating lymphocytes (TILs).
  • NK Cell Expansion – Compatible for dual-use applications (optional IL-15/IL-21 supplementation).

 

Validated T-Cell Expansion Protocol

Parameter Details
Cell Type Human peripheral blood T cells
Medium KBM591 (+ IL-2 supplementation)
Vessel Anti-CD3-coated 24-well plate
Seeding Density 4×10⁵ cells/well
Culture Duration 14 days at 37°C, 5% CO₂
Outcome High-density T cell proliferation

 

Fig. 3: NK cell Proliferative Response

 

Fig. 2: CD3 Positive Rate

 

Validated NK Cell Expansion Protocol

Parameter Details
Cell Type Human peripheral blood T cells
Medium KBM591 (+ IL-2 supplementation)
Vessel NKCC-c-coated 24-well plate
Seeding Density 1×10^6 cells/well
Culture Duration 14 days at 37°C, 5% CO₂
Outcome High-density NK cell proliferation

 

 

Fig. 3: NK cell Proliferative Response

 

Fig. 4: NK cell Marker Ratio

 

Documents

KBM591 English

KBM591 Protocol English