KBM591 – Advanced Serum-Free T-Cell Expansion Media
Optimized for High-Yield T Cell Activation & Expansion
KBM591 is a serum-free, xeno-free cell culture medium specifically engineered for high-efficiency T cell expansion, while also supporting NK cell culture. Ideal for immunotherapy development and lymphocyte research, this versatile formulation delivers consistent, high-density T-cell growth without animal-derived components.
KBM591 is a RUO product.
Key Benefits for T-Cell Expansion
✔ Superior T Cell Proliferation – Achieve robust expansion rates for human peripheral blood T cells in controlled conditions.
✔ Serum-Free & Xeno-Free – Eliminates batch variability and reduces reliance on autologous plasma, lowering costs while ensuring reproducibility.
✔ Ready-to-Use or Customizable – Use as a standalone medium or supplement with serum/cytokines (e.g., IL-2) for tailored applications.
✔ Antibiotic-Free Flexibility – Add only the antibiotics or additives your protocol requires.
✔ Regulatory-Friendly – RUO product with a defined formulation, reducing risks for preclinical workflows.
✔ Validated Performance – Proven in 14-day T cell expansion protocols (see data below).
Recommended Applications:
- CAR-T/TCR-T Therapy Development – Scale T cell production for immunotherapy pipelines.
- T Cell Research – Study activation, exhaustion, or tumor-infiltrating lymphocytes (TILs).
- NK Cell Expansion – Compatible for dual-use applications (optional IL-15/IL-21 supplementation).
Validated T-Cell Expansion Protocol
Parameter | Details |
---|---|
Cell Type | Human peripheral blood T cells |
Medium | KBM591 (+ IL-2 supplementation) |
Vessel | Anti-CD3-coated 24-well plate |
Seeding Density | 4×10⁵ cells/well |
Culture Duration | 14 days at 37°C, 5% CO₂ |
Outcome | High-density T cell proliferation |


Validated NK Cell Expansion Protocol
Parameter | Details |
---|---|
Cell Type | Human peripheral blood T cells |
Medium | KBM591 (+ IL-2 supplementation) |
Vessel | NKCC-c-coated 24-well plate |
Seeding Density | 1×10^6 cells/well |
Culture Duration | 14 days at 37°C, 5% CO₂ |
Outcome | High-density NK cell proliferation |

