Publications

Article Snippet: “Mononuclear cells were isolated from blood samples by density gradient centrifugation using Ficoll–Paque PLUS (GE Healthcare, Little Chalfont, UK), and T lymphocytes were expanded by culture in interleukin-2 (IL-2)-containing KBM-502 medium (Kohjin Bio, Saitama, Japan)…”

Article Snippet: “The PBMCs were cultured for 10 days in KBM502 (Kohjin Bio).”

Article Snippet: “On the 3rd day, the PBMCs were replated on mitomycin C (MMC)-treated mouse STO feeder cells and cultured in KBM502.“

Sawada, T., Chater, T.E., Sasagawa, Y. et al.

Developmental excitation-inhibition imbalance underlying psychoses revealed by single-cell analyses of discordant twins-derived cerebral organoids.

Mol Psychiatry 25, 2695–2711 (2020).

DOI: https://doi.org/10.1038/s41380-020-0844-z

Article Snippet: “The transfected cells were then seeded onto 100-mm dishes covered with mitomycin C-treated SNL76/7 feeder cells (ATCC, VA, USA) in KBM502 (Kohjin Bio, Saitama, Japan) supplemented with 16.7 μl/10⁶ cells of Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific, MA, USA).”

Qian, E., Uemura, M., Kobayashi, H. et al.

A human induced pluripotent stem cell model from a patient with hereditary cerebral small vessel disease carrying a heterozygous R302Q mutation in HTRA1.

Inflamm Regener 43, 23 (2023).

DOI: https://doi.org/10.1186/s41232-023-00273-7

Article Snippet: “For T lymphocytes expansion and activation, the cells were cultured for 7 days with KBM502 (Kohjin Bio, Saitama, Japan) with Dynabeads Human T-Activator CD3/CD28…”

Yamashita, K., Iwatsuki, M., Yasuda-Yoshihara, N. et al.

Trastuzumab upregulates programmed death ligand-1 expression through interaction with NK cells in gastric cancer.

Br J Cancer 124, 595–603 (2021).

DOI: https://doi.org/10.1038/s41416-020-01138-3

Article Snippet: “To maintain NK cell viability and activity, KBM 502 (Kohjin Bio Co., Saitama, JP) which is a medium that contains human IL-2, was used for the co-culture assays.”

Yamamoto, G., Miyabe, I., Tanaka, K. et al.

SVA retrotransposon insertion in exon of MMR genes results in aberrant RNA splicing and causes Lynch syndrome.

Eur J Hum Genet 29, 680–686 (2021).

DOI: https://doi.org/10.1038/s41431-020-00779-5

Article Snippet: “These cells were resuspended in KBM502 (KOHJIN BIO, Sakado, Japan) supplemented with 10% FBS (GE Healthcare) and penicillin–streptomycin (FUJIFILM Wako Pure Chemical, Osaka, Japan), and plated and cultured in tissue culture tubes (TPP, Trasadingen, Switzerland) at 37 °C in a 5% CO₂ humidified atmosphere.”

Article Snippet: “The PBMCs were cultured for 7 days in KBM502 (Kohjin Bio) and reprogrammed using the Sendai virus (CytoTune-iPS 2.0 Sendai Reprogramming kit, Thermo Fisher Scientific) according to the manufacturer’s protocol.”

Article Snippet: “Two days after infection, PBMCs were transferred onto mitomycin-C treated mouse STO cells and cultured in KBM502 (Kohjin Bio).”

Article Snippet: “After 24 h of infection, the medium was changed to fresh KBM 502 medium (KOHJIN BIO, Saitama, Japan), and the cells were collected and split at 5×10⁴ cells into 10 cm plates pre-seeded with mouse embryonic fibroblasts (MEFs). “