The Exoid is Izon’s latest instrument for measuring the physical characteristics of nanoparticles in solution. Based on principles of Tunable Resistive Pulse Sensing (TRPS), the Exoid enables precise measurements of particle size, concentration and zeta potential, using a suitably sized nanopore that enables measurements within a defined size range. The uniquely high-resolution data that can be obtained using the Exoid enables you to compare your samples with confidence.

What is Tunable Resistive Pulse Sensing (TRPS)?

Tunable Resistive Pulse Sensing (TRPS) is a single-particle measurement technique designed for measuring nanoparticles in the size range of 40 nm to 11 µm. 

TRPS monitors current flow through a tunable nanopore. Particles crossing the nanopore cause transient changes in the flow of an ionic current, which can be detected and analysed via the resulting blockades. Each particle size is determined for individual particles; blockade magnitude is proportional to particle size, and particle concentration is calculated from the particle flow rate measured at several different applied pressures. Zeta potential (a measure of the effective surface charge) is derived by measuring electrophoretic mobility, which is calculated based on the speed at which the particle traverses the nanopore.

Unlike ensemble techniques such as the dynamic light scattering (DLS), where larger particles can skew size measurements, the Exoid provides insightful, high-resolution measurements that allow you to detect important differences in particle size distribution. Given the wide size range that can be analysed using different sized nanopores, TRPS has been adopted for use across a range of disciplines and industries. The Exoid is suited for use in the analysis of nanoparticles including exosomes and other extracellular vesicles, lipid nanoparticles, virus-like particles, and monoclonal antibody preparations.

Why Choose TRPS Over NTA?

In contrast with the true single-particle nature of TRPS, Nanoparticle Tracking Analysis (NTA) uses the tracking of particle motion via light scattering to assess the mean squared displacement of particles moving under Brownian motion, in a sample chamber illuminated by a laser beam. Particle concentration calculations are based on the number of particles tracked in an estimated illumination volume. The resolution capabilities of NTA are also relatively lower that TRPS. 

Particle size distribution of a quadrimodal mixture. TRPS (top) has a better resolution capability compared to NTA (bottom). 

Upload and provide link to this Youtube video explaining TRPS: What is Tunable Resistive Pulse Sensing (TRPS)? (youtube.com)

Specification:

• Analysis range: 40 nm to 11 µm
• Concentration range: 1E5 to 1E11 particles/mL (size-dependent)
• Compatible electrolyte solutions: Include PBS, HEPES, MES and KCI
• Weight: 10.8 kg
• Footprint: 300 x 300 mm
• Height: 250 mm
• Certification: ISO 13485:2016

How do I incorporate TRPS in my research?

You may purchase the Exoid or get in touch with us ([email protected]) to discuss a lease agreement.