Izon qEV Concentration Kit
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Depending on the downstream method of choice, output volumes from qEV columns may require a concentration step prior to analysis. The qEV Concentration Kit enables EV-containing samples to be reduced to a convenient, concentrated form for downstream processing.
- Enrich intact EVs that have been isolated using qEV columns.
- Concentrate a range of collection volumes. EV concentration can be achieved for purified collection volumes ranging from 600 µL to 20 mL.
- Reduce your sample to a convenient form for downstream processing. Specialised Nanotrap® particles are used to convert your sample to a pellet, which can be reconstituted to a volume that suits.
- Compatible with downstream analysis such as western blotting, mass spectrometry, or RNA analysis following extraction with Izon’s qEV RNA Extraction Kit.
Contains 5 mL Nanotrap® particles. The volume of required Nanotrap® particles depends on the volume of purified collection volume to be concentrated.
| qEV Column used for purification | Purified Collection Volume (PCV) (mL) | Required volume of Nanotrap® EV Capture Particles (µL) |
|---|---|---|
| qEVsingle | 0.60-0.80 | 50.0 |
| qEVoriginal | 1.5-3.0 | 100.0 |
| qEV2 | 6.0-8.0 | 150.0 |
| qEV10 | 10.0-20.0 | 200.0 |
Reference Publications
Meng, Yingchao et al.
Direct isolation of small extracellular vesicles from human blood using viscoelastic microfluidics
Science advances vol. 9,40 (2023): eadi5296.
Article Snippet: “sEV samples isolated from both the microfluidic device and UC were first concentrated using the qEV Concentration Kit (Izon Science, Christchurch, New Zealand). Specifically, sEV samples were incubated with Nanotrap EV Particles (NEVPs; Izon Science, Christchurch, New Zealand) at room temperature for 2 hours and then centrifuged at 10,000g for 10 min to form pellets.”
Evelyn Lattmann, Luca Räss, Marco Tognetti, et al.
Size-exclusion chromatography combined with DIA-MS enables deep proteome profiling of extracellular vesicles from melanoma plasma and serum
Cellular and Molecular Life Sciences. 81.
DOI: 10.1007/s00018-024-05137-y
Article Snippet: “100 µL of concentration beads (qEV Concentration Kit, IZON) were added to 2 mL of fraction 7–10 and incubated on a rotating wheel for 1h at 22°C.”
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