The 11B11 antibody binds to mouse Interleukin-4 (IL-4), a 14 kDa cytokine that is largely secreted by activated T cells of the Th2 subset, and to some degree by NKT and mast cells. This cytokine acts as a stimulatory factor for B cells, inducing their proliferation and differentiation, as well as playing a role in immunoglobulin class-switching. IL-4 may also provide autocrine stimulation for T cells, and affect the function of antigen presenting cells such as macrophages and dendritic cells. IL-4 can bind and signal via three cell surface receptor types: CD124 by itself, CD124 in combination with the common gamma chain (type I complex), or CD124 combined with CD213a1 (type II complex).

The 11B11 antibody is widely used for detection of intracellular levels of IL-4 protein by flow cytometry, as well as for analysis of soluble cytokine as measured by ELISA, and in functional assays to neutralization cytokine-receptor interactions. Please choose the appropriate format for each application.

Recent Publications:
Onizawa M, Oshima S, Schulze-Topphoff U, Oses-Prieto JA, Lu T, Tavares R, Produhomme T, Duong B, Whang MI, Advincula R, Agelidis A, et al. 2015. Nat Immunol. 16(6): 618-627. (in vitro activation)

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