HypoGuard Cell Transport Media
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HypoGuard Cell Transport Media
Advanced Transport Media for Mammalian Cells – Maintain Viability & Function for Up to 120 Hours
Transporting mammalian cells without compromising viability is now easier than ever. Our chemically defined transport media is specifically designed to maintain cell health for up to 120 hours at 2-8°C, making it ideal for short-term storage or transport across labs or countries. Tested on MSCs and trusted by leading researchers and clinicians, our product is a game-changer for cell therapy and regenerative medicine applications.
Key Features
- Ready-to-Use: No pre-preparation required – just open and use.
- Xeno-free & Protein-Free: Ensures compatibility with sensitive cell types and reduces contamination risks.
- Maintains Cell Viability for up to 120 Hours: Ensure your cells arrive healthy and functional, even during extended transport.
- Eliminates Dry Ice Shipments: Save costs and simplify logistics by removing the need for dry ice.
- Proven on MSCs: Rigorously tested on Mesenchymal Stem Cells, ensuring reliability for critical applications, also suitable for other sensitive cells.
- Easy to Integrate: No additional equipment or workflow changes needed – seamlessly fits into your existing protocols.
- Ideal for cell therapy, research, and clinical applications (GMP Grade Available).
Benefits
- Reduce cell loss during transport and storage.
- Say goodbye to the cost and logistical challenges of dry ice shipments
- Ensure consistent results in your experiments or therapies.
Applications
- Cell Therapy: Ensure therapeutic cells arrive in optimal condition for patient treatment.
- Regenerative Medicine: Maintain the viability of stem cells and other critical cell types.
- Research: Simplify cell transport and storage for lab-to-lab collaborations.
Ready to streamline your cell transport process? Request a free sample of Hypoguard Cell Transport Media today and experience the difference!
For more information, please contact [email protected].
Supporting Data
Mesenchymal Stem Cells (MSCs) are highly versatile and have been clinically used to treat various diseases, including musculoskeletal disorders, graft-versus-host disease, Crohn’s disease, and even cancer (Wu et al., 2020). The culture, expansion, and preservation processes of these cells are crucial to ensure their optimal performance. HypoGuard preserves the viability of MSCs (>95%) for up to 5 days (120 hours), and maintains cell concentration for up to 3 days (72 hours; Figure 1A). Importantly, after 120 hours of hypothermic storage, cell recovery remained optimal (Figure 1B) with minimal changes in doubling time after 24 and 120 hours of storage (Figure 1C).
Figure 1: Storage of human Mesenchymal Stem Cells in HypoGuard.(A) MSCs were harvested in suspension and stored in HypoGuard for up to 120 hours at 4°C. The graph shows the mean ± SD(n=3)of the concentration (no. of live cells/uL)and the cell viability of MSCs at each indicated time point. (B)Image of replated cells after 120 hours in storage.(C) At 24 hours and 120 hours post-storage, the cells were plated at 5000 cells/cm2 and cultured for 4 days to determine the doubling time. The graph represents the mean ± SDof three biological replicates.
Peripheral blood mononuclear cells (PBMCs), the immune cell fraction of blood, are commonly isolated for in-vitro studies. These cells are highly sensitive to environmental changes and can lose viability if not handled correctly.Figure 2 demonstrate that HypoGuard can maintain cell viability above 70% for up to 120 hours at 4°C, with minimal reduction in the concentration of viable cells.
Figure 2: Storage of human peripheral blood mononuclear cells in HypoGuard. PBMCs were harvested in suspension and stored in HypoGuard for up to 120 hours. The graph shows the mean ± SD (n=3) of the concentration(no. of live cells/uL)and thecell viability of PBMC sat each indicated time point
Protocol
- For adherent cells, detach cells from the culture surface using trypsin and transfer them to a suitable centrifuge tube. For suspension cells, aliquot cells into a centrifuge tube
- Centrifuge the cells to form a pellet.
- Discard the supernatant or spent culture medium.
- Resuspend the cell pellet in an appropriate volume of HypoGuard(recommended concentration: 1-5 x 10^6 cells/mL) and store at 2-8°C.
Product Specification
| Product Name | Hypoguard Hypothermic Transport Medium |
| SKU | AS-9013 |
| Volume | 100 mL |
| Storage Temperature | 2-8° C |
