Human Interleukin 1 Beta (IL1b) ELISA Kit
Human Interleukin 1 Beta (IL1b) ELISA Kit
Human IL1b (Interleukin 1 Beta) sandwich ELISA Kit specific for IL1b in cosmetic and personal care products research & development.
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Overview of Human IL1b (Interleukin 1 Beta)
| Product name: | Human IL1b(Interleukin 1 Beta) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin |
| Assay Type: | Sandwich |
| Sensitivity: | 5.8 pg/mL |
| Standard: | 1000 pg/mL |
| Range: | 15.63-1000 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Cytokine; Tumour immunity; Infection immunity; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta(IL1b). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 1 Beta(IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Interleukin 1 Beta(IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Beta(IL1b) in the samples is then determined by comparing the OD of the samples to the standard curve. |
[accordions]
[accordion title= “Standard Curve“]
| Concentration (ng/mL) | OD | Corrected OD |
| 1000.00 | 2.207 | 2.125 |
| 500.00 | 1.613 | 1.531 |
| 250.00 | 1.115 | 1.033 |
| 125.00 | 0.757 | 0.675 |
| 62.50 | 0.421 | 0.339 |
| 31.25 | 0.297 | 0.215 |
| 15.63 | 0.195 | 0.113 |
| 0.00 | 0.082 | 0.000 |
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[accordion title= “Precision“]
Intra-assay Precision (Precision within an assay)： CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)： CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. [/accordion] [accordion title= "Recovery“]
Matrices listed below were spiked with a certain level of recombinant IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of IL1b in samples.
| Matrix | Recovery range | Average |
| serum(n=5) | 92-105% | 98% |
| EDTA plasma(n=5) | 83-95% | 89% |
| Heparin plasma(n=5) | 79-93% | 86% |
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[/accordion]
[accordion title=”Linearity“]
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-93% | 91-103% | 82-99% | 99-104% |
| EDTA plasma(n=5) | 84-99% | 81-95% | 98-107% | 96-101% |
| Heparin plasma(n=5) | 86-105% | 84-98% | 95-102% | 88-106% |
[/accordion]
[accordion title=”Publications“]
- https://www.frontiersin.org/articles/10.3389/fonc.2021.622727/full
- https://www.sciencedirect.com/science/article/abs/pii/S002432052030597X?via%3Dihub
- https://www.sciencedirect.com/science/article/pii/S0753332221008544
- https://www.sciencedirect.com/science/article/pii/S2667031321000038?via%3Dihub
- https://jintensivecare.biomedcentral.com/articles/10.1186/s40560-020-00486-9
- https://www.tandfonline.com/doi/full/10.1080/21655979.2022.2076388
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