Human COL1(Collagen Type I) ELISA Kit
Human COL1(Collagen Type I) ELISA Kit
Human COL1(Collagen Type I) ELISA Kit is Sandwich enzyme immunoassay made for cosmetics & personal care products research & development.
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| Product name: | Human COL1(Collagen Type I) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | COL1; COLI |
| Assay Type: | Sandwich |
| Sensitivity: | 26.8 pg/mL |
| Standard: | 4000 pg/mL |
| Range: | 62.5-4000 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Metabolic pathway;Developmental science;Bone metabolism; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I(COL1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type I(COL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I(COL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I(COL1) in the samples is then determined by comparing the OD of the samples to the standard curve. |
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[accordion title= “Standard Curve“]
| Concentration (ng/mL) | OD | Corrected OD |
| 4000.00 | 2.235 | 2.140 |
| 2000.00 | 1.712 | 1.617 |
| 1000.00 | 1.225 | 1.130 |
| 500.00 | 0.937 | 0.842 |
| 250.00 | 0.587 | 0.492 |
| 125.00 | 0.396 | 0.301 |
| 62.50 | 0.197 | 0.102 |
| 0.00 | 0.095 | 0.000 |
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[accordion title= “Precision“]
Intra-assay Precision (Precision within an assay)：CV%<8%
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Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
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Inter-assay Precision (Precision between assays)：CV%<10%
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Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
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[accordion title= “Recovery“]
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Matrices listed below were spiked with a certain level of recombinant COL1 and the recovery rates were calculated by comparing the measured value to the expected amount of COL1 in samples.
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| Matrix | Recovery range | Average |
| serum(n=5) | 92-107% | 99% |
| EDTA plasma(n=5) | 80-95% | 87% |
| Heparin plasma(n=5) | 85-97% | 91% |
[/accordion]
[accordion title=”Linearity“]
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-102% | 83-96% | 79-93% | 88-97% |
| EDTA plasma(n=5) | 88-103% | 86-92% | 91-99% | 95-102% |
| Heparin plasma(n=5) | 79-91% | 87-96% | 83-101% | 82-95% |
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[accordion title=”Publications“]
https://onlinelibrary.wiley.com/doi/abs/10.1111/wrr.13006
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