Human AGR2(Anterior Gradient Protein 2) ELISA Kit
Human AGR2(Anterior Gradient Protein 2) ELISA Kit
The Human AGR2(Anterior Gradient Protein 2) ELISA Kit is a is Sandwich enzyme immunoassay suitable for cosmetic & personal care product research.
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Human AGR2(Anterior Gradient Protein 2) ELISA Kit Overview
| Product name: | Human AGR2(Anterior Gradient Protein 2) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | GOB4; HAG2; XAG2; AG2; PDIA17; HPC8; Protein Disulfide Isomerase Family A,Member 17; Secreted cement gland protein XAG-2 homolog |
| Assay Type: | Sandwich |
| Sensitivity: | 0.62 ng/mL |
| Standard: | 100 ng/mL |
| Range: | 1.57-100 ng/mL |
| Sample Type: | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Tumor immunity;Reproductive science;Bone metabolism; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anterior Gradient Protein 2(AGR2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anterior Gradient Protein 2(AGR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anterior Gradient Protein 2(AGR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anterior Gradient Protein 2(AGR2) in the samples is then determined by comparing the OD of the samples to the standard curve. |
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[accordion title= “Standard Curve“]
| Concentration (ng/mL) | OD | Corrected OD |
| 100.00 | 2.121 | 2.020 |
| 50.00 | 1.755 | 1.654 |
| 25.00 | 1.269 | 1.168 |
| 12.50 | 0.886 | 0.785 |
| 6.25 | 0.525 | 0.424 |
| 3.13 | 0.352 | 0.251 |
| 1.57 | 0.193 | 0.092 |
| 0.00 | 0.101 | 0.000 |
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[accordion title= “Precision“]
Intra-assay Precision (Precision within an assay)：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. [/accordion] [accordion title= "Recovery“]
Matrices listed below were spiked with certain level of recombinant AGR2 and the recovery rates were calculated by comparing the measured value to the expected amount of AGR2 in samples.
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| Matrix | Recovery range | Average |
| serum(n=5) | 88-102% | 95% |
| EDTA plasma(n=5) | 78-92% | 85% |
| Heparin plasma(n=5) | 80-97% | 88% |
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[accordion title= “Linearity“]
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AGR2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-104% | 82-94% | 86-96% | 81-97% |
| EDTA plasma(n=5) | 86-95% | 91-106% | 87-100% | 95-101% |
| Heparin plasma(n=5) | 85-94% | 87-101% | 89-99% | 85-95% |
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