Biotium products are distributed only in Singapore and Thailand.
Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a mixed live-dead population and also used as a counterstain in multicolor fluorescent assays.
SIZE |
CATALOG #
Â
|
PRICE
Â
|
1 mL | #40010 |
$183 |
0.5 mL (2 mM in DMSO) |
#40044 |
$198 |
PRODUCT ATTRIBUTES
CAS number |
61926-22-5 |
---|---|
DNA/RNA dye |
DNA & RNA dye |
Cellular localization |
Nucleus & cytoplasm |
Cell permeability |
Membrane impermeant |
Detection method/readout |
Fluorescence microscopy, Flow cytometry |
Apoptosis/viability marker |
Dead cell stain |
Colors |
Red |
Excitation/Emission |
528/617 nm (with DNA) |
Molecular weight |
857 |
PRODUCT DESCRIPTION
Reference Publications
Leung, Ada W Y et al. Combined Use of Gene Expression Modeling and siRNA Screening Identifies Genes and Pathways Which Enhance the Activity of Cisplatin When Added at No Effect Levels to Non-Small Cell Lung Cancer Cells In Vitro PloS one vol. 11,3 e0150675. DOI: 10.1371/journal.pone.0150675 Article Snippet: “At 72 hours post-treatment, the cells were stained for 20 minutes with dye exclusion marker Hoechst 33342 (16.2 μM, Invitrogen, Carlsbad, CA) and the nuclear stain ethidium homodimer I (EthD-I; 1μM, Biotium, Hayward, CA) and then imaged via the IN Cell Analyzer 1000″  Scialis, Renato J, and José E Manautou. Elucidation of the Mechanisms through Which the Reactive Metabolite Diclofenac Acyl Glucuronide Can Mediate Toxicity The Journal of pharmacology and experimental therapeutics vol. 357,1 (2016): 167-76. DOI: 10.1124/jpet.115.230755 Article Snippet: “Ethidium homodimer-1 was purchased from Biotium Inc. (Hayward, CA). All LC–MS/MS solvents were of high analytical grade and were purchased from Burdick and Jackson (Muskegon, MI).”  Tao Jiang, et al. Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics Biofabrication 12 015024 DOI: 10.1088/1758-5090/ab3a5c Article Snippet: “MCTSs viability was determined using calcein-AM (AAT Bioquest, Inc) and ethidium homodimer-1 (EthD-1, Biotium) assay. Calcein-AM is a cell-permeant component that enters the cells and is cleaved by esterases inside the living cells, producing an intense green fluorescence.”