Name: Dulbecco's Phosphate Buffered Saline (DPBS) 10X w/o Calcium w/o Magnesium 10L
SKU: X0515-C10LS
Availability: InStock

High-Quality Phosphate Buffered Saline (PBS) for Cell Culture

Biowest offers premium phosphate buffered saline (PBS) solutions, specifically designed to maintain cell integrity and support various laboratory procedures. Our PBS products are rigorously tested and quality-controlled to ensure optimal performance.

Key Benefits:

Sterility: Ensures a contamination-free environment for your cell cultures.
Physiologically balanced: Provides a suitable environment for a wide range of mammalian cells.
Calcium and magnesium-free: Compatible with trypsin-based dissociation methods.
Versatility: Ideal for cell enumeration, washing, resuspension, and chromatography.
Long shelf life: Maintain product integrity for up to 48 months.

Trust Biowest for your cell culture essentials.

We deliver Biowest solutions across South East Asia (Singapore, Thailand, Vietnam, Indonesia, Philippines, Myanmar) and Across China Only.

Reference Publications

Widowati, W., Gunanegara, R. F., Rizal, R., Widodo, W. S., Amalia, A., Wibowo, S. H. B., Handono, K., Marlina, M., Lister, I. N. E., & Chiuman, L.

Comparative analysis of Wharton’s jelly mesenchymal stem cell (WJ-MSCS) isolated using explant and enzymatic methods.

Journal of Physics Conference Series, 1374(1), 012024. (2019).

https://doi.org/10.1088/1742-6596/1374/1/012024

Article Snippet: “The UC was washed with PBS (Biowest, X0515- 500) supplemented with antibiotic and transferred to laboratory using transport medium containing phosphate buffer saline (PBS) (Biowest, X0515-500)…”

Ginting, C., Lister, I. E., Girsang, E., Artie, D., Aviani, J., & Widowati, W.

Apoptotic, MDA, and FGF2 level of quercitrin treatment on hypoxic-induced EA.hy926 Cell Line.

Journal of Reports in Pharmaceutical Sciences, 10(1), 22. (2021).

DOI: https://doi.org/10.4103/jrptps.jrptps_70_20

Article Snippet: “Cells were washed with PBS 1X (Biowest X0515-500) and harvested with trypsin-EDTA (0.25-0.038%) (Biowest, L0931-500).”

Da Hyun Lee, Jeong Su Park, Yu Seol Lee, Soo Han Bae
PERK prevents hepatic lipotoxicity by activating the p62-ULK1 axis-mediated noncanonical KEAP1-Nrf2 pathway
Redox Biology, Volume 50, 2022, 102235, ISSN 2213-2317,
DOI: https://doi.org/10.1016/j.redox.2022.102235

Article Snippet: “The cells were washed with Dulbecco’s phosphate-buffered saline (Biowest, X0515-500), fixed with 4% paraformaldehyde (Biosesang, P2031) for 30 min, and washed three times with PBS.”

Widowati, W., Jasaputra, D. K., Heriady, Y., Wahyudianingsih, R., Laksmitawati, D. R., Faried, A., Kusuma, H. S. W., Zahiroh, F. H., Rizal, R., Ginting, C. N., & Lister, I. N. E.

Case study: Heterogeneity of and CD44+/CD24- cancer stem cell subpopulation of breast cancer patients in Bandung, Indonesia

Trends in Sciences, 21(7), 7437. (2024).

DOI: https://doi.org/10.48048/tis.2024.7437

Article Snippet: “All participants had signed the informed consent form. The specimens were immediately treated with 1% Gentamicin (Gibco, 15750060), 1% Antibiotic-Antimycotic (Ab-Am) (Biowest, L0010-100), 1% Amphotericin B (Amp B)and Nanomycopulitine (Biowest, LX16-100) addition after being washed with PBS (phosphate buffer saline) 1×(Biowest X0515-500).”

Ylä-Anttila, P., & Masucci, M. G.

Inhibition of selective autophagy by members of the herpesvirus ubiquitin-deconjugase family.

Biochemical Journal, 478(12), 2297–2308. (2021).

DOI: https://doi.org/10.1042/bcj20210225

Article Snippet: “Cells were harvested 48 h after transfection, resuspended in PBS (Biowest, X0515-500) containing protease inhibitor cocktail and samples were snap frozen and stored at −20°C.”

Ylä-Anttila, P., & Masucci, M. G.

Inhibition of selective autophagy by members of the herpesvirus ubiquitin-deconjugase family

Biochemical Journal, 478(12), 2297–2308 (2021b)

DOI: https://doi.org/10.1042/bcj20210225

Ylä-Anttila, P., Gupta, S., & Masucci, M. G.

The Epstein-Barr virus deubiquitinase BPLF1 targets SQSTM1/p62 to inhibit selective autophagy

Autophagy, 17(11), 3461–3474 (2021)

DOI: https://doi.org/10.1080/15548627.2021.1874660

Article Snippet: “Cell pellets were resuspended in PBS (Biowest, X0515-500) containing protease inhibitor cocktail. Samples were snap frozen and stored at −20°C.”

De La Torre, N. G., Fernández-Durango, R., Gómez, R., Fuentes, M., Roldán-Pallarés, M., Donate, J., Barabash, A., Alonso, B., Runkle, I., Durán, A., Rubio, M. A., & Calle-Pascual, A. L.

Expression of angiogenic MicroRNAs in endothelial progenitor cells from type 1 diabetic patients with and without diabetic retinopathy

Investigative Ophthalmology & Visual Science, 56(6), 4090. (2015)

DOI: https://doi.org/10.1167/iovs.15-16498

Article Snippet: “Peripheral blood mononuclear cells (PBMNCs) were isolated from 40 mL peripheral blood by Ficoll gradient centrifugation, washed three times with PBS (Dulbecco’s phosphate buffer, X0515; Biowest, Nuaillé, France), and plated at a density of 106 cells/cm² on human fibronectin-coated flasks (0172003; Tebubio, Le-Perray-en-Yvelines, France).”

Lesmana, S. B., Djuanda, R., & Sugiaman, V. K.

Cytotoxicity test of apple cider vinegar as a root canal irrigant against fibroblast cells

Deleted Journal, 9(2), 158. (2022)

DOI: https://doi.org/10.30659/odj.9.2.158-167

Article Snippet: “The culture medium in the T25 flask (Corning, 430168) was discarded, and then rinsed with PBS (Biowest, X0515-500) 1 time as much as 2 ml to remove the remaining medium.”