Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, CF® Dye Conjugates, Single Label for STORM
Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, CF® Dye Conjugates, Single Label for STORM
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Product Description
This is a highly cross-adsorbed donkey anti-mouse IgG (H+L) secondary antibody that has a low degree of labeling (DOL) with one of our bright and photostable CF® dyes.
- CF® dye single label secondary antibody ideal for STORM imaging
- Highly cross-adsorbed for specific staining with minimal background
- Available in 7 bright and photostable CF® dyes
Secondary antibodies with a low DOL, or number of dye molecules per antibody molecule, have been reported to be optimal for STORM (Bittel et al. (2015) Proc. SPIE 9331). This product is prepared by single labeling (DOL=1) of highly cross-adsorbed goat anti-mouse IgG (H+L) with a selection of compatible CF® dyes for (d)-STORM super-resolution microscopy. To minimize cross-reactivity, the antibody has been adsorbed against human, bovine, horse, rabbit, and swine serum.
Learn more about CF® Dyes for super-resolution microscopy.
CF® Dye Secondary Antibodies, Single Label for STORM
| Dye (Ex/Em) | Donkey Anti-Goat | Donkey Anti-Guinea Pig | Donkey Anti-Mouse | Donkey Anti-Rabbit | Goat Anti-Mouse | Goat Anti-Rabbit |
|---|---|---|---|---|---|---|
| Cross-adsorption | Ck, GP, Hs, Hu, Ms, Rb, Rt, SHm | Bv, Ck, Gt, Hs, Hu, Ms, Rb, Rt, Shp, SHm | Bv, Ck, Gt, GP, Hs, Hu, Rb, Shp, SHm | Bv, Ck, Gt, GP, Hs, Hu, Ms, Rt, Shp, SHm | Bv, Hs, Hu, Rb, Sw | Hu, Ms, Rt |
| CF®535ST (535/568 nm) |
20823 | 20824 | 20821 | 20822 | ||
| CF®568 (562/583 nm) |
20836 | 20838 | 20802 | 20803 | 20800 | 20801 |
| CF®594ST (593/614 nm) |
20806 | 20807 | 20804 | 20805 | ||
| CF®647 (650/665 nm) |
20829 | 20837 | 20806 | 20811 | 20808 | 20809 |
| CF®660C (667/685 nm) |
20815 | 20816 | 20812 | 20813 | ||
| CF®680 (681/698 nm) |
20819 | 20820 | 20817 | 20818 | ||
| CF®750 (755/777 nm) |
20827 | 20828 | 20825 | 20826 |
PRODUCT ATTRIBUTES
| Reactivity (target) | Mouse IgG |
|---|---|
| Clonality | Polyclonal |
| Host species | Donkey |
| Cross adsorption | Bovine, Chicken, Goat, Guinea pig, Horse, Human, Rabbit, Sheep, Syrian hamster |
| Antibody type | Secondary |
| Concentration | 1 mg/mL |
| Species reactivity | Mouse |
| Applications | STORM |
Format:
1 mg/mL in PBS, 50% glycerol, 2 mg/ml BSA, 0.05% sodium azide
Bv: bovine; Ch: chicken; Gt: goat; GP: guinea pig; Hs: horse; Hu: human; Ms: mouse; Rb: rabbit; Sh: sheep; SHm: Syrian hamster; Sw: swine; Rt: rat
CF® dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.
CF is a registered trademark of Biotium, Inc.
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