Product Description

Empower your research with DMEM High Glucose, a versatile cell culture medium specially formulated to support the growth and proliferation of a wide range of mammalian cells.

Optimized for Efficiency:

• High Glucose: Ideal for cells with high energy demands.
  • Glucose concentration: 4.5 g/L
• Stable Glutamine: Ensures consistent quality and cell performance.
• Sodium Pyruvate: Enhances metabolic activity and cell viability.

Seamlessly Integrate into Your Workflow:

• Ready-to-use: Save time and resources with this pre-formulated solution.
• Available in various sizes: Choose the right volume for your needs.
• Customizable: Contact us to create a bespoke media formulation tailored specifically for your research.

DMEM High Glucose is just one of the many tools we offer to help you translate your research from bench to bed. Explore our comprehensive portfolio of cell culture solutions and discover how we can support your journey towards groundbreaking discoveries.

We deliver Biowest solutions across South East Asia (Singapore, Thailand, Vietnam, Indonesia, Philippines, Myanmar) and Across China Only.

Product Details

Reference Publications

Konstantopoulos G, Leventakou D, Saltiel DR, Zervoudi E, Logotheti E, Pettas S, Karagianni K, Daiou A, Hatzistergos KE, Dafou D, Arsenakis M, Kottaridi C.

HPV16 E6 Oncogene Contributes to Cancer Immune Evasion by Regulating PD-L1 Expression through a miR-143/HIF-1a Pathway. Viruses.

Viruses vol. 16,1 113. 12 Jan. 2024

doi:10.3390/v16010113

Articles Snippet: “SiHa, CaSki and HEK293T cell lines were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA) and grown in Dulbecco’s Modified Eagle medium (DMEM, Biowest #L0103) supplemented with 10% fetal bovine serum (FBS, Biowest #S1810) (Gibco/Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 1% L-Glutamine (Biowest #X0550) and 1% Penicillin-Streptomycin (Biowest #L0022)…”

Maldonado-Pérez N, Tristán-Manzano M, Justicia-Lirio P, Martínez-Planes E, Muñoz P, Pavlovic K, Cortijo-Gutiérrez M, Blanco-Benítez C, Castella M, Juan M, Wenes M, Romero P, Molina-Estévez FJ, Marañón C, Herrera C, Benabdellah K, Martin F.

Efficacy and safety of universal (TCRKO) ARI-0001 CAR-T cells for the treatment of B-cell lymphoma

Frontiers in immunology vol. 13 1011858. 6 Oct. 2022, doi:10.3389/fimmu.2022.1011858

Articles Snippet: “HEK-293T (ATCC^® CRL-11268) cells were maintained in DMEM (Biowest) supplemented with 10% FBS (Gibco) and 1% P/S (Biowest) at 37°C and 10% CO₂ atmosphere.”

Hwang S, Cho J-M, Yoon Y-J, Seo S, Hong Y and Lim J-Y

Retroductal dexamethasone administration promotes the recovery from obstructive and inflammatory salivary gland dysfunction

Front. Immunol. 15:1418703.

DOI: https://doi.org/10.3389/fimmu.2024.1418703

Article Snippet: “Bone marrow cells were collected and cultured on a suspension plate in DMEM (# L0103–500, Biowest, France) …”

L. Iovino, J. VanderZwaag, G. Kaur, M. Khakpour, V. Giusti, M. Donadon, A. Chiavegato, L. Tenorio-Lopes, E. Greggio, M.E. Tremblay, L. Civiero

Investigation of microglial diversity in a LRRK2 G2019S mouse model of Parkinson’s disease

Neurobiology of Disease, Volume 195, 2024, 106481, ISSN 0969-9961

DOI: https://doi.org/10.1016/j.nbd.2024.106481

Article Snippet “Then, the tissues were transferred to 15 mL Falcon tubes containing 1 mL of Dulbecco’s Modified Eagle Medium (DMEM, Biowest, #L0103) supplemented with 10% FBS (Corning) …”

Park, J., Park, H., Lee, Y.L., Weon, S., Kim, Y., Yang, J., and Kim, T.

Blocking TNFα attenuates progressive cartilage matrix degradation in inflammatory arthritis.

Experimental and Therapeutic Medicine, 22, 808.

DOI: https://doi.org/10.3892/etm.2021.10240

Article Snippet: “Hyaline cartilage from OA knee joints was scraped using a rongeur and collected in serum-free DMEM (cat. no. L0103-500; Biowest) buffer containing 1 mg/ml Collagenase type 2 (cat. no. C6885; Sigma-Aldrich).”

Ulloa, M., Macías, F., Clapp, C. et al.

Prolactin is an Endogenous Antioxidant Factor in Astrocytes That Limits Oxidative Stress-Induced Astrocytic Cell Death via the STAT3/NRF2 Signaling Pathway.

Neurochem Res 49, 1879–1901 (2024).

DOI: https://doi.org/10.1007/s11064-024-04147-3

Article Snippet: “The supernatant was discarded and the pellet resuspended in dulbecco’s modified eagle medium (DMEM, cat. no. L0103; Biowest, MO, USA)…”

Kim, O., Tran, P.T., Gal, M., Lee, S.J., Na, S.H., Hwangbo, C., & Lee, J.

RAS‑stimulated release of exosomal miR‑494‑3p promotes the osteolytic bone metastasis of breast cancer cells

International Journal of Molecular Medicine, 52, 84. (2023).

DOI: https://doi.org/10.3892/ijmm.2023.5287

Article Snippet: “The MDA-MB-231, RAW264.7 (ATCC TIB-71) murine macrophages, and C2C12 (ATCC CRL-1772) murine myoblast cell lines were cultured in DMEM (BioWest, #L0103-500) supplemented with 10% FBS and 1% penicillin/streptomycin.”

P. A. Virych, V. A. Chumachenko, P. A. Virych, V. O. Pavlenko & N. V. Kutsevol.

Cytotoxicity of dextran-graft-polyacrylamide/zinc oxide nanoparticles against doxorubicin-resistant breast cancer cells

The Ukrainian Biochemical Journal 95:6, pages 73-80. (2023)

DOI: https://doi.org/10.15407/ubj95.06.073

Article Snippet: “Cells were maintained in plastic flasks (SPL) in DMEM (L0103, Biowest) supplemented with 10% fetal calf serum (Biowest)…”

Documents

MSDS

Technical Data Sheet

Composition