Biotium products are distributed only in Singapore and Thailand.

Visualize Cell Dynamics with DiO: The Classic Lipophilic Tracer

Biotium’s DiO stain offers a reliable and versatile option for labeling cell membranes in your research. This green fluorescent, lipophilic carbocyanine dye is a well-established tool for tracing cellular movement and interactions.

Key Features of DiO:

• Widely Used Tracer: DiO is a popular choice for lipophilic tracing experiments due to its well-documented performance and ease of use.
• Bright Green Fluorescence: The λEx/λEm maxima at 484/501 nm provides clear and distinct labeling for accurate image analysis.
• Compatible with DiI: Combine DiO with DiI for dual-color labeling and colocalization studies, expanding your experimental capabilities.
• Multiple Formats: Choose from the versatile DiO powder or the ready-to-use CellBrite™ Green solution for simplified workflow integration.

Please note:

• DiO exhibits lower solubility and a slower lateral diffusion rate compared to newer alternatives like Neuro-DiO.
• For applications requiring superior solubility and faster diffusion, consider exploring Neuro-DiO (Product #60015).

DiO Applications:

• Live cell imaging of cell migration and morphology
• Investigating cell-cell interactions
• Monitoring neurite outgrowth
• Multicolor colocalization studies (in combination with DiI)