CXCL16 belongs to the CXC chemokine family and conducts signaling through the CXCR6 receptor. CXCL16 may act as an attractant of lymphocyte subsets during inflammation process and may facilitate certain immune responses. Among six cysteine residues of the chemokine domain, there are four highly conserved cysteine residues characteristic of CXC chemokines. The CXCL16 gene encodes a 273 amino acid polypeptide, which contains a 29 amino acid cytoplasmic domain and a 20 amino acids transmembrane sequence.

Sequence: 
NQGSVAGSCSCDRTISSGTQIPQGTLDHIRKYLKAFHRCPFFIRFQLQSKSVCGGSQDQWVRELVDCFERKECGTGHGKSFHHQKHLP with polyhistidine tag at the N-terminus

Source:
Escherichia coli

Endotoxin Test:
<0.1 EU per 1 μg of the protein by the LAL method. Activity:
Measure by its ability to chemoattract BaF3 cells transfected with mouse CXCR6. The ED₅₀ for this effect is <3 ng/mL. Purity:
>98% as determined by SDS-PAGE. Ni-NTA chromatography

Formulation:
The protein was lyophilized from a solution containing 1X PBS, pH 7.4.

Reconstitution:
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Storage:
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

Note:
Please use within one month after protein reconstitution.

Data Sheet