CryoEase-PF Global Cell Cryoperservation & Transport Media
CryoEase-PF Global Cell Cryoperservation & Transport Media
$420.00
Add to cart
CryoEase-PF a DMSO-Free and Protein-Free Cryopreservation for MSCs & Sensitive Cells – Transport Cells with Confidence
Maintain MSC Viability at Subzero & Hypothermic Temperatures For Reliable Nationwide & International Shipping
Bridge geographical and long-distance cell transport boundaries with CryoEase-PF, the next-generation cryopreservation solution engineered for seamless shipments across expansive regions like Thailand, Vietnam, Australia, Canada, U.S, Russia and China. Eliminate the complexities of traditional cold chains and ensure your mesenchymal stem cells (MSCs) arrive with optimal viability and functional integrity. CryoEase-PF is your key to reliable, cross-border scientific or cross-country collaboration.
This DMSO-free, protein-free formulation ensures your sensitive cells like MSCs maintain their viability, integrity, and functional potential, whether stored at subzero (frozen) or hypothermic (4-10°C) temperatures. Ideal for researchers and clinicians seeking reliable cell preservation and transportation solutions, CryoEase-PF simplifies your workflow and enhances clinical outcomes.
Key Features:
- DMSO-Free, Protein-Free Formulation: Eliminates the toxicity and variability associated with traditional cryopreservation methods. Designed to minimise downstream processing to reduce post-thaw cell loss.
- Dual-Temperature Compatibility (4-10°C & Subzero): Offers unparalleled flexibility for diverse transit conditions. Protects cells at both subzero and hypothermic temperatures, offering flexibility for long-term storage and hypothermic storage at 4–10°C, crucial for vast geographical regions.
- Enhanced Viability: Minimizes ice crystal formation and maintains osmotic balance, preventing cellular damage. CryoEase-PF is formulated to ensure over 70% cell viability and recovery up to 3 days post-thaw, minimizing cell loss and enhancing usability, ensuring consistent cell quality upon arrival.
- Functional Integrity: Preserves the therapeutic potential of sensitive cells like MSCs or even modified MSCs, ensuring uncompromised, consistent and reliable therapeutic applications.
- GMP-Compliant Materials: Formulated with GMP-compliant materials facilitating a seamless transition from research to clinical applications, even across international borders.
Benefits:
- Reliable Nationwide & International Shipping: Ensures cell viability across vast distances, from coast-to-coast in the US or across the Australian outback.
- Consistent Cell Quality: Maintains cell viability and function, regardless of transit duration or environmental conditions. Provides a stable microenvironment, protecting cells from oxidative and metabolic stress.
- Reduced Cold Chain Dependency: Minimizes the need for liquid nitrogen or dry ice, simplifying logistics and reducing shipping costs for short-term delivery requirements.
- Optimized for MSCs & modified MSCs: Tailored to the unique structural and metabolic needs of mesenchymal stem cells.
- Streamlined Collaboration: Enables seamless cell exchange between labs and institutions across large countries and continents.
Applications:
- Nationwide & International Research Collaborations: Enables seamless cell exchange between labs across vast geographical regions.
- Global Clinical Trials: Ensures consistent cell quality for multi-site studies spanning continents.
- Biobanks and International Biobanking: Ensures long-term viability of stored MSCs without compromising quality. Secures long-term storage and distribution of MSCs across borders and within large countries.
- Research Laboratories: Protects valuable MSCs during experiments and transfers between labs across countries.
- Veterinary Medicine: Preserves MSCs for animal therapies across expansive regions.
Ensure the integrity of your cells across any distance with CryoEase-PF. Contact us at [email protected] today to optimize your cell cryopreservation and transport logistics.
| Volume | 100ml |
|---|
Supporting Data
To demonstrate the versatility of CryoEase-DF, we stored human adipose-derived MSCs at a density of 5 x 106 cells/mL. Upon thawing, the cells were stored at 4°C for up to 72 hours. Cell viability was assessed by 7AAD staining. Cells stored in CryoEase-PF retained >85% viability after 72 hours as compared to other commercially available formulations with <75% viability.
It is critical that the cells are not just viable but remain expandable in culture. MSCs frozen in CryoEase-PF were thawed and plated immediately (Acute Thaw) or stored at 4°C for up to 72 hours before being plated at 5,000 cells/cm2. The population doubling time (PDT) was determined after 4 days. Remarkably, cells stored in CryoEase-PF retain a PDT of approx. 23 hours and 30 hours when plated immediately or stored for 72 hours.
To ensure that MSC identity is not compromised post-storage, MSCs were subjected to phenotyping in accordance with the criteria set forth by the International Society for Cell and Gene Therapy (ISCT) (Dominici et. al.). MSC stored in CryoEase-PF retained both their surface marker profile and trilineage differentiation potential.
Sample Protocol
- Trypsinise cells from monolayer.
- Quantify cell number and viability.
- Pellet cells by centrifugation.
- Resuspend in an appropriate amount of CryoEase-PF.
- We recommend a freezing density of 1-5 x 106 cells/mL
- Transfer to cryovials.
- Equilibrate at room temperature for 10-15 mins.
- Store at -80C.
- Optional: use control rate freezer/freezing devices for -1C/min freezing
