CryoEase-PF hMSC DMSO & Protein Free Cryoperservation Media
CryoEase-PF hMSC DMSO & Protein Free Cryoperservation Media
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CryoEase-PF A DMSO-Free, Protein-Free Cryopreservation Media for MSCs & Sensitive Cells
Preserve >85% Viability, Expansion Capacity & Potency – From Cryobank to Bedside
CryoEase-PF is the only dual temperature compatibility, DMSO-free, protein-free cryopreservation medium proven to maintain >85% MSC viability post-thaw with uncompromised expansion potential and phenotype, meeting ISCT criteria for clinical-grade MSCs. This media is developed by leading cell therapy developers in Singapore for global cell therapy developers for allogeneic/autologous MSC production, global trials, and biobanking.
Why CryoEase-PF Outperforms Commercial Alternatives
1. Clinically Superior Viability & Recovery
✅ >85% Viability After 72h at 4°C (vs. <75% in competing formulations)
✅ 23-Hour Population Doubling Time (PDT) post-thaw – near-identical to fresh MSCs
✅ 30-Hour PDT Even After 72h Hypothermic Storage – ensures expandability after shipping delays
Validation Data:
- Cell Type: Human adipose-derived MSCs (5 x 10⁶ cells/mL)
- Storage: Cryopreserved at -150°C → Thawed → Stored at 4°C for 72h
- Viability: >85% (7AAD staining)
- Phenotype: Retained CD73+/CD90+/CD105+ and CD34-/CD45-/HLA-DR- (ISCT-compliant)
2. Unmatched Functional Integrity
✔ Preserved Trilineage Differentiation (Osteogenic, Chondrogenic, Adipogenic)
✔ Stable Surface Marker Profile – Critical for IND/EMA filings
✔ Consistent Performance Across Batches – Pre-mixed, endotoxin-tested, GMP-compliant
3. Global Logistics Simplified
- Dual-Temperature Stability:
- Long-term: Vapor-phase LN₂ (-150°C)
- Short-term: 4°C for 72h (ideal for multi-site trials)
- Reduced Cold Chain Burden: Eliminates dry ice for regional transport
Key Features of CryoEase vs Competitors
| Parameter | CryoEase-PF | Commercial Alternatives |
| 72h Post-Thaw Viability | >85% | <75% |
| Population Doubling Time | 23h (acute thaw), 30h (72h delay) | 35–50h (variable) |
| ISCT Compliance | Full retention of phenotype & differentiation | Often compromised |
| Toxicity Risks | DMSO-free, xeno-free | DMSO-dependent |
Applications for MSC-Based Therapies
- Preclinical R&D: Ensure translational relevance with clinically mimicked storage conditions.
- Phase I–III Trials: Maintain MSC potency across global trial sites (US, EU, APAC).
- Allogeneic Cell Banking: Scale production with high-recovery, low-attrition cryopreservation.
- International Collaborations: Ship MSCs at 4°C without significant viability loss.
Now Expanding to New Cell Types – Join Our Validation Network
We Invite Researchers to Collaborate!
Are you working with sensitive cell lines that challenge conventional cryopreservation? Partner with us to:
✓ Test CryoEase-PF in your specific application
✓ Access complimentary samples for pilot studies
✓ Explore co-development opportunities
Be among the first to test CryoEase-PF on your specialized cell lines – Contact our team for collaborative opportunities or sample requests!
Email: [email protected]
| Volume | 100ml |
|---|
Supporting Data
To demonstrate the versatility of CryoEase-DF, we stored human adipose-derived MSCs at a density of 5 x 106 cells/mL. Upon thawing, the cells were stored at 4°C for up to 72 hours. Cell viability was assessed by 7AAD staining. Cells stored in CryoEase-PF retained >85% viability after 72 hours as compared to other commercially available formulations with <75% viability.
It is critical that the cells are not just viable but remain expandable in culture. MSCs frozen in CryoEase-PF were thawed and plated immediately (Acute Thaw) or stored at 4°C for up to 72 hours before being plated at 5,000 cells/cm2. The population doubling time (PDT) was determined after 4 days. Remarkably, cells stored in CryoEase-PF retain a PDT of approx. 23 hours and 30 hours when plated immediately or stored for 72 hours.
To ensure that MSC identity is not compromised post-storage, MSCs were subjected to phenotyping in accordance with the criteria set forth by the International Society for Cell and Gene Therapy (ISCT) (Dominici et. al.). MSC stored in CryoEase-PF retained both their surface marker profile and trilineage differentiation potential.
Sample Protocol
Cryopreservation Protocol
- Trypsinize cells from a monolayer.
- Quantify cell number and viability.
- Pellet cells by centrifugation.
- Resuspend in an appropriate amount of CryoEase-PF.
- We recommend a freezing density of 1-5 x 106 cells/mL
- Transfer to cryovials.
- Equilibrate at room temperature for 10-15 mins.
- Store at -80C.
- Optional: use control rate freezer/freezing devices for -1C/min freezing
Post-Thaw Sample Protocol
- Thaw vials in 37 °C water bath (or use automated thawing device – eg, ThawStar)
- Add complete media/appropriate thawing buffer at a 1:5 ratio (eg, 4 mL media to 1 mL frozen thawed cell suspension)
- Quantify cell number and viability.
- The sample is ready for downstream use. No spinning is required.
