Reference Publications

Qin, Siyue et al.

Transcriptional factor Nrf2 is essential for aggresome formation during proteasome inhibition

Biomedical reports vol. 11,6 (2019): 241-252.

DOI: https://doi.org/10.3892%2Fbr.2019.1247

Article Snippet: “Cell death-inducing effects of proteasomal inhibition were measured with CF488A-Annexin V (ANXA5) and propidium iodide (PI) Apoptosis kits (cat. no. 30061; Biotium, Inc.) as described previously. A total of 100,000 cells/cm² were grown in 10 cm dishes. After 24 h, they were treated with DMSO (0.1%) or 2 µM MG132 (in DMSO) for 20 h.”

Kaya-Tilki, Elif, and Miriş Dikmen.

Neuroprotective effects of some epigenetic modifying drugs’ on Chlamydia pneumoniae-induced neuroinflammation: A novel model

PloS one vol. 16,11 e0260633.

DOI: 10.1371/journal.pone.0260633

Article Snippet: “To determine neuronal apoptosis associated with Aβ exposure, 25×10³ differentiated SH-SY5Y cells were seeded in 24-well plates and Aβ was applied. After 24 h, cells were trypsinized and staining was performed according to the CF 488A Annexin V and PI Apoptosis Kit (Biotium, USA, Cat. No: 30061) protocol.”

Chenliang Zhang, Ju Gao, Mengen Li, Yongkang Deng and Changan Jiang.

p38δ MAPK regulates aggresome biogenesis by phosphorylating SQSTM1 in response to proteasomal stress

Journal of Cell Science (2018) 131, jcs216671.

DOI: 10.1242/jcs.216671

Article Snippet: “Cell death assay was performed with CF488A-Annexin V (ANXA5) and propidium iodide (PI) apoptosis Kit (Biotium, 30061) as described previously. Briefly, cells grown on 10 cm dishes were treated with DMSO or 2 µM MG132 for 18 h, then harvested by digestion with 0.05% trypsin-EDTA solution.”