CellGem Single Cell Isolation and Culture Microfluidics Slide

CellGem Single Cell Isolation and Culture Microfluidics Slide

CellGem Single Cell Isolation and Culture Microfluidics Slide

CellGem is a simple and smart single cell isolation and culture platform. It comprises of an array of microwells to capture single cells & an array of culture wells for stable single cells line development.

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Accelerate Your Single-Cell Research with CellGem

Struggling with inefficient workflows and low cell viability? CellGem offers a high-throughput, user-friendly solution to overcome these challenges. Our innovative microfluidic technology enables precise single-cell capture and cultivation, empowering you to unlock groundbreaking discoveries.

Key Benefits:

  • High-throughput isolation: Efficiently capture and culture numerous single cells simultaneously.
  • Exceptional cell viability: Maintain cell health and integrity throughout the process.
  • Versatile applications: Suitable for monoclonal antibody production, cancer research, and gene editing.
  • User-friendly design: Simple operation and minimal hands-on time.
  • Cost-effective: Optimize reagent usage and reduce experimental costs.

 

How CellGem Works:

Our platform features an array of microwells for capturing individual cells, followed by an array of culture wells for long-term cultivation. This integrated design streamlines your workflow and enhances experimental reproducibility.

 

Validated for a Wide Range of Cell Lines:

CellGem has been successfully tested with various cell types, including CHO-K1, AA8, 293T, U2OS, NIH3T3, A549, hybridoma, and MCF7, ensuring compatibility with your specific research needs.

Innovative single cell culture

User Manual

Download User Manual

Basic CellGem user manual

 

Step 1: Pre Rinse

 

Step 2: Cell Loading & Wash

 

Step 3: Seal, Flip & Culture Cells

 

Step 4: Change Single Cell Culture Media

 

Step 5: Harvest Single Cell Cultures

FAQ

Q: What are the advantages of CellGem® compared to other single-cell separation products in the market?

  • CellGem® does not require complex instrumentation and is easy to use. It also has high single-cell capture efficiency, high single-cell growth rate, easy to-identify single cells, and consumes minimal cell culture medium.

Q:If there are cells existing in the channel, will it affect the monoclonality of my single cell-derived colony? If so, how do I perform the subsequent cell harvesting steps to avoid contaminating my single cell colonies?

  • Cells that exist in the channel do have the potential to contaminate your single-cell colonies and affect monoclonality. So, It’s important to ensure that the washing step is done well to remove excess cells from the chip.If residual cells still exist in the channel, do not directly inject trypsin into the chip before the harvesting stage. Instead, disassemble the chip while the cells are still attached, then individually add trypsin to each culture well to detach the cells. The working volume of each culture well is 1.5 μL.

Q:Following the previous question, what do you mean by high single cell growth rate? In theory, single cells don’t grow into colonies efficiently, why is this?

  • Because all the cells inside the CellGem® chip share the same pool of culture medium and also share secreted growth factors between each other. Each single cell still owns its isolated growth space at the same time.(Depend on cell type.)

Q: Is there a contamination risk of using sealing tape to seal the chip inlets?

  • The sealing tape used in the kit is tested and certified to be sterile. It’s safe to use with cell culture.

Q: An air bubble was trapped in the chip during the priming step. How do I get rid of it?

  • Fully aspirate the priming solution from the chip, then rapidly inject the priming solution back into the chip. This helps to remove bubbles.

Q:Why is my cell capture efficiency low? How do I increase single cell capture efficiency?

  • If your cells are clumpy, then they cannot be efficiently captured by the

    capture wells. We recommend trying a cell dissociation reagent that’s compatible with your cells (ex. Accumax, sorting buffer.)

    2. There may be excess bubbles in the chip, affecting cell capture

    efficiency. We recommend trying the bubble removal method on page 8 of the handbook 

    3. You may have selected a capture well size that’s not a good fit for your cell of interest.

    We recommend measuring the size (diameter) of your cells and selecting the product with the corresponding suitable capture well size 

    4. Your cell concentration may be too low. Cell concentration of 1*106

    cell/ml or above is recommended.

     

    5. Loading cells multiple times to increase capture efficiency.

Q: After flipping over the chip, how do the captured cells accurately land in their corresponding culture wells?

  • The capture wells are specifically designed to ensure that each captured single cell will only land in its designated culture well after flipping the chip. However, the chip can only be flipped over once. Users should avoid flipping it over again after the single-cell transfer step.

Reference Publications

Chen HH, Nguyen TV, Shih YH, Chang KC, Chiu KC, Hsia SM, Fuh LJ, Shieh TM.

Combining microfluidic chip and low-attachment culture devices to isolate oral cancer stem cells.

J Dent Sci. 2024 Jan; 19(1):560-567.

DOI: https://doi.org/10.1016/j.jds.2023.10.005

Article Snippet: “Single-cell isolation and culture from parental HSC3, OECM-1, and SAS cell lines were performed using a microfluidic chip (CellGem®, OriGem Biotechnology Inc., Taichung, Taiwan).