Biotium products are distributed only in Singapore and Thailand.
Elevate Your Labeling Experiments with Aminooxy Biotin
Discover the superior performance of Aminooxy Biotin for stable and efficient labeling of aldehydes and ketones. Our high-quality reagent offers a reliable alternative to hydrazides, ensuring precise and reproducible results.
Key Benefits:
- Stable Oxime Linkage: Forms a robust bond with aldehydes and ketones, at neutral/acidic pH, ensuring long-lasting labeling. The reaction is fast and can be further accelerated using a catalyst like aniline.
- Versatile Applications: Label polysaccharides, glycoproteins, antibodies, and cell-surface sialic acid groups.
- Excellent Compatibility: Works seamlessly with CF® Dye Aminooxy derivatives for fluorescent labeling. These next-generation dyes offer superior brightness, photostability, and water solubility compared to traditional fluorescent dyes.
Applications:
- Polysaccharides labeling
- Glycoprotein labeling
- Antibody labeling
- Cell surface sialic acid groups labeling on living animal cells
How to label glycoproteins with a detectable tag?
- Introduce aldehyde groups into the glycoprotein by mild periodate oxidation
- Treat the functionalised proteins with an aminooxy reagent
- Aminooxy labeling of antibody glycosylation sites can be used as an alternative to succinimidyl ester labeling of amines, for antibodies where amine labeling affects the antibody binding affinity.
Choose Aminooxy Biotin for:
- Enhanced labeling stability
- Precise and reproducible results
- Versatility in various applications
Order your Aminooxy Biotin today and experience the difference!
Reference Publications
Reference Publications
Matta, C., Boocock, D.J., Fellows, C.R. et al.
Molecular phenotyping of the surfaceome of migratory chondroprogenitors and mesenchymal stem cells using biotinylation, glycocapture and quantitative LC-MS/MS proteomic analysis
Sci Rep 9, 9018 (2019).
DOI: https://doi.org/10.1038/s41598-019-44957-y
Article Snippet: “The glycocapture method with aminooxy-biotin yielded a very high relative amount of surfaceome proteins compared to non-surfaceome proteins;…”