AccuGreen™ Broad Range dsDNA Quantitation Kit
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A fluorescent dsDNA quantitation kit with a linear range of 2-1000 ng DNA. Designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher.
Product Description
The AccuGreen™ Broad Range dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples. The kit provides a linear detection range from 2 – 1000 ng and is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher. The AccuGreen™ Broad Range assay is also available without a DNA standard (31070), for those that wish to use their own standard.
Features
- Designed specifically for Thermo’s Qubit® fluorometer
- Linear range: 2 – 1000 ng dsDNA
- Sample concentration range: 0.1 to 1000 ng/uL
- Green fluorescence emission
- Replaces Qubit® dsDNA BR Assay Kit for cost savings
Kit components
- AccuGreen™ Broad Range DNA Quantitation Solution, 1X
- AccuGreen™ Standard 1 (0 ng/uL)
- AccuGreen™ Standard 2 (100 ng/uL)
AccuGreen™ for the Qubit® fluorometer
The AccuGreen™ Broad Range dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher.
The linear range of the AccuGreen™ BR assay is 2 to 1000 ng of DNA per tube. DNA samples with concentrations between 0.1 and 1000 ng/uL may be quantified using sample volumes between 1 and 20 uL (for example, 1 uL of 1000 ng/uL is 1000 ng total, and 20 uL of 0.1 ng/uL is 2 ng total, which will both fall within the linear range of the assay). If you use the most common sample volume of 10 uL, the starting sample concentration should be 0.2 to 100 ng/uL.
Fluorescence-Based dsDNA Quantitation
AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.
Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.
All DNA Quantitation Kits
| dsDNA Quantitation Kit | Linear detection range (dsDNA in assay) |
Ex/Em (nm) | Suggested Instrument | Features |
|---|---|---|---|---|
| AccuGreen™ High Sensitivity | 0.1-100 ng | 502/523 | Qubit® fluorometer | Compare to the Qubit® dsDNA HS assay from Thermo Fisher |
| AccuGreen™ Broad Range | 2-1000 ng | 500/530 | Qubit® fluorometer | Compare to the Qubit® dsDNA BR assay from Thermo Fisher Non-toxic & non-mutagentic DNA quantitation dye |
| AccuBlue® NextGen | 1 pg-3 ng* | 468/507 | Fluorescent Plate Reader | Most sensitive assay available for quantitation of precious or dilute samples Optimal for sensitive applications such as NGS or digital PCR * Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity |
| AccuClear® Ultra High Sensitivity | 30 pg-250 ng | 468/507 | Fluorescent Plate Reader | Versatile kit with high sensitivity and wide linear range |
| AccuBlue® High Sensitivity | 0.2-100 ng | 485/530 | Fluorescent Plate Reader | Non-toxic & non-mutagentic DNA quantitation dye |
| AccuBlue® Broad Range | 2-2000 ng | 350/460 | Fluorescent Plate Reader | Broad linear range with blue fluorescence |
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit is a registered trademark of Thermo Fisher Scientific.
References
BioRxiv (2019) doi:10.1101/2019.12.11.860585
Product Attributes
| Excitation/Emission | 500/530 nm (with DNA) |
|---|---|
| Storage Conditions | Store at 2 to 8 °C |
Protocols
SDS
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