A fluorescent dsDNA quantitation kit with a linear range of 1-3,000 pg DNA, an unprecedented level of sensitivity in dsDNA detection. Designed for use in a fluorescence microplate reader.

The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA, with a linear range from ~1 – 3,000 pg (depending on instrument capability). This assay is designed for use with fluorescence microplate readers. The AccuBlue® NextGen assay is also available without any DNA standards (31061), for those that wish to use their own standard.

With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue® NextGen outperforms rivals such as PicoGreen® in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue® NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.

Because the AccuBlue® NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see table below), assay volume, and pipet accuracy.

Plate Reader Detection Limits

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA Quantitation Kits