Biotium products are distributed only in Singapore and Thailand.
5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).
- Off-white solid soluble in DMSO
- Store desiccated at 4°C
- C25H16O9
- MW: 460
- [79955-27-4]
PRODUCT ATTRIBUTES
CAS number | 79955-27-4 |
---|---|
Applications | pH indicators |
Cell permeability | Membrane permeant |
Cellular localization | Nucleus & cytoplasm |
pKa | pKa 6.5 |
Indicator type | Ratiometric |
Colors | Green |
Excitation/Emission | 495/519 nm (pH 9) (after hydrolysis) |
REFERENCES
1. J Cell Sci 98, 343(1991).
2. Nature 295, 524 (1982).
3. J Immunol Meth 100, 261 (1987).
4. J Immunol Meth 33, 33 (1980).