Njikọ nke Annexin V

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Annexin V fluorescent conjugates nwere ike iji chọpụta mkpụrụ ndụ apoptotic. Annexin V na-egbochi mgbochi mmadụ bụ 35-36 kDa calcium na-adabere na phospholipid na-ekekọta protein nke nwere njikọ dị elu maka phosphatidylserine (PS). N'ime mkpụrụ ndụ nkịtị, PS dị na mpempe akwụkwọ dị n'ime nke akpụkpọ ahụ cytoplasmic. N'ime mkpụrụ ndụ apoptotic, Otú ọ dị, a na-atụgharị PS site n'ime ime gaa na mpụta mpempe akwụkwọ plasma, ebe enwere ike ịchọpụta ya site na fluorescently akara Annexin V.

Chọpụta mkpụrụ ndụ apoptotic na ngwa ngwa, nke a pụrụ ịdabere na ya nke mpụga phosphatidylserine
Njikọ nwere nnukwu nhọrọ nke CF® agba agba, gbakwunyere biotin, R-PE, APC, na ihe ndị ọzọ na-esiji agba fluorescent.
Ihe e ji esiji ákwà Biotium's CF® nwere nchapụta dị elu, ike foto, ma na-agbaze mmiri
Enwere ike iji maka microscopy fluorescence, onyonyo cell ndụ, na cytometry eruba

A na-enye CF® Dye, biotin, na FITC conjugates nke Annexin V dị ka 50 ug/mL ngwọta na 10 mM Tris, 1 mM EDTA, 30 mM NaCl, 1 mg/mL bovine serum albumin, 0.1% sodium azide, pH 7.5. R-PE na APC Annexin V conjugates na-enye na PBS, 5 mg/mL BSA, 0.05% sodium azide na 5 uL kwa ule.

Njikọ nke Annexin V — **Pịa ka ilele oge gafechara.**
Onyonyo nke apoptosis n'ime mkpụrụ ndụ HeLa nke staurosporine mebere nwere ntụpọ CF®594 Annexin V (red, apoptotic cell surface probe) na Oxazole Yellow (green, nwụrụ anwụ cell ntụpọ nuklia). Nuclei niile nwere ntụ na-acha anụnụ anụnụ na Hoechst.

Chọpụta Apoptosis

Anyị na-enyekwa Annexin V Conjugates, Azide-free na Lyophilized na agba sitere na UV ruo na-acha uhie uhie maka ntụpọ cell dị ndụ, na Nso-IR CF® Dye Annexin V (nakwa lyophilized na enweghị nchekwa), dakọtara na obere anụmanụ NIR na vivo onyonyo. Annexin V dịkwa na nyocha ndị ọzọ na anyị Ngwa Apoptotic na Necrotic Staining Kit. Hụ n'uju anyị nhọrọ nke Nleba anya cell na nyocha apoptosis.

Agba agba CF® kacha mma

A haziri agba agba Biotium nke ọgbọ na-abịa CF® ka ọ bụrụ nke nwere ike ịsacha mmiri nke ukwuu yana uru dị na nchapụta na fotostability atụnyere Alexa Fluor®, DyLight®, na akwa fluorescent ndị ọzọ. Mụtakwuo maka CF® Dyes.

Njikọ nke Annexin V

Product	Ọpụ/Em	Mmasị	size	Katalọgụ Mba
Biotin Annexin V	N / A	50 μg/ml	0.5 mL	Ịzụta 29013
CF®350 Annexin V	347/448 nm	50 μg/ml	0.5 mL	Ịzụta 29012
CF®405M Annexin V	408/452 nm	50 μg/ml	0.5 mL	Ịzụta 29009
CF®450 Annexin V	450/538 nm	50 μg/ml	0.5 mL	Ịzụta 29083
CF®488A Annexin V	490/515 nm	50 μg/ml	0.5 mL	Ịzụta 29005
CF®555 Annexin V	555/565 nm	50 μg/ml	0.5 mL	Ịzụta 29004
CF®568 Annexin V	562/583 nm	50 μg/ml	0.5 mL	Ịzụta 29010
CF®583R Annexin V	586/609 nm	50 μg/ml	0.5 mL	Ịzụta 29085
CF®594 Annexin V	593/614 nm	50 μg/ml	0.5 mL	Ịzụta 29011
CF®633 Annexin V	630/650 nm	50 μg/ml	0.5 mL	Ịzụta 29008
CF®640R Annexin V	642/662 nm	50 μg/ml	0.5 mL	Ịzụta 29014
CF®647 Annexin V	650/665 nm	50 μg/ml	0.5 mL	Ịzụta 29003
CF®660R Annexin V	663/682 nm	50 μg/ml	0.5 mL	Ịzụta 29069
R-PE Annexin V	496, 546, 565/578 nm	5 uL/nnwale	100 l	Ịzụta 29045-100uL
R-PE Annexin V	496, 546, 565/578 nm	5 uL/nnwale	500 l	Ịzụta 29045-500uL
APC Annexin V	650/660 nm	5 uL/nnwale	100 l	Ịzụta 29057-100uL
APC Annexin V	650/660 nm	5 uL/nnwale	500 l	Ịzụta 29057-500uL
FITC Annexin V	496/516 nm	50 μg/ml	0.5 mL	Ịzụta 29001
Ihe nchekwa 5X Annexin V	N / A	5X	15 mL	Ịzụta 99902

CF bụ ụghalaahịa edenyere n'akwụkwọ ikikere nke Biotium, Inc. Alexa Fluor, na DyLight bụ ụghalaahịa edenyere n'akwụkwọ ikikere nke Thermo Fisher Scientific.

Ondrej Zenata, Zdenek Dvorak, Radim Vrzal

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Mpempe akwụkwọ edemede: "E nwetara Neomycin sulfate site na Amara (Kraków, Poland). Annexin V Enwetara ihe nchekwa ihe na Annexin V-CF488A conjugate sitere na Biotium (Fremont, CA, USA)."

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Mpempe akwụkwọ edemede: "A na-eme nyocha nke Annexin V⁶⁴^,⁶⁵ site na mbụ na-ekewa cortical organoids na monolayer neurons, bụ ndị emechara maliteghachi na Annexin V binding buffer (Invitrogen) na Annexin V-CF488A njikọ (Biotium, Inc., Hayward, CA, USA) na Hoechst 33342 (Chemometec) ma tinye ya maka nkeji 15 na 37°C."

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Mpempe akwụkwọ edemede: pụta ìhè site na Tebụl nyocha azụmahịa dị oke mkpa "Biotium Mix-n-Stain Kit maka CF488A Biotium Cat#92253, Biotium Mix-n-Stain Kit maka CF568 Biotium Cat#92255, Annexin V - CF488A Conjugates Biotium Anya #29005. "

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DOI: https://doi.org/10.1007/s43440-022-00407-7

Mpempe akwụkwọ edemede: "Annexin V-CF488A njikọ na Annexin V a na-ekekọta ihe nchekwa sitere na Biotium Inc. (USA). Ihe mgbochi: mgbochi Mcl-1 (4572, mgbochi polyclonal nke na-achọpụta ọkwa nke mmadụ Mcl-1, isi iyi: oke bekee)"

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DOI: 10.21037/tcr.2018.03.31

Mpempe akwụkwọ edemede: "Annexin V na-ekekọta ihe nchekwa na Annexin V-CF488A njikọ sitere na Biotium (Fremont, USA). LightCycler 480 Probes Master sitere na Roche Diagnostic Corporation"