5-CFDA (5-Carboxyfluorescein diacetate, single isomer)

5-CFDA (5-Carboxyfluorescein diacetate, single isomer)

5-CFDA (5-Carboxyfluorescein diacetate, single isomer)

$162.00

$162.00

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Biotium products are distributed only in Singapore and Thailand.

5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).

  • Off-white solid soluble in DMSO
  • Store desiccated at 4°C
  • C25H16O9
  • MW: 460
  • [79955-27-4]

PRODUCT ATTRIBUTES

CAS number 79955-27-4
Applications pH indicators
Cell permeability Membrane permeant
Cellular localization Nucleus & cytoplasm
pKa pKa 6.5
Indicator type Ratiometric
Colors Green
Excitation/Emission 495/519 nm (pH 9) (after hydrolysis)

REFERENCES

1. J Cell Sci 98, 343(1991).

2. Nature 295, 524 (1982).

3. J Immunol Meth 100, 261 (1987).

4. J Immunol Meth 33, 33 (1980).

PROTOCOLS

PI-CFDA

SDS

SDS 51018