PE-Cyanine7 Anti-Mouse CD4 (GK1.5)
Add to cart
The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.
The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will “compete” for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.
Product Details
| Name | PE-Cyanine7 Anti-Mouse CD4 (GK1.5) |
|---|---|
| Cat. No. | 60-0041 |
| Alternative Names | L3T4, T4 |
| Gene ID | 12504 |
| Clone | GK1.5 |
| Isotype | Rat IgG2b, κ |
| Reactivity | Mouse |
| Cross Reactivity | Syrian Hamster |
| Format | PE-Cyanine7 |
| Application | Flow Cytometry |
| Citations* | Rita Kansal, Noah Richardson, Indira Neeli, Saleem Khawaja, Damian Chamberlain, Marium Ghani, Qurat-ul-ain Ghani, Louisa Balazs, Sarka Beranova-Giorgianni, Francesco Giorgianni, James N. Kochenderfer, Tony Marion, Lorraine M. Albritton, Marko Radic. Sustained B cell depletion by CD19-targeted CAR T cells is a highly effective treatment for murine lupus. Sci Transl Med. 2019 Mar 6;11(482). pii: eaav1648. doi: 10.1126/scitranslmed.aav1648
Uzodinma U. Uche, Ann R. Piccirillo, Shunsuke Kataoka, Stephanie J. Grebinoski, Louise M. D’Cruz, and Lawrence P. Kane. PIK3IP1/TrIP restricts activation of T cells through inhibition of PI3K/Akt. J Exp Med. 2018 Dec 3;215(12):3165-3179. doi: 10.1084/jem.20172018 Taniguchi RT, DeVoss JJ, Moon JJ, Sidney J, Sette A, Jenkins MK, and Anderson MS. 2012. Proc. Natl. Acad. Sci. 109: 7847-7852. (in vivo depletion) Wolkers MC, Gerlach, C, Arens R, Janssen EM, Fitzgerald P, Schumacher TN, Medema JP, Green DR, and Schoenberger SP. 2012. Blood. 119:798-804. (in vivo depletion) Lee L-F, Logronio K, Tu GH, Zhai W, Ni I, Mei L, Dilley J, Yu J, et al. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry). Thornton EE, Looney MR, Bose O, Sen D, Sheppard D, Locksley R, Huang X, and Krummel MF. 2012. J. Exp. Med. 10:1084. (Immunohistochemistry – OCT embedded frozen tissue) Stephen TL, Wilson BS, and Laufer TM. 2012. Proc. Natl. Acad. Sci. 109: 7415-7420. (Immunoprecipitation) Hammerbeck CD and Hooper JW. 2011. J. Virol. 85(19) 9929-9944. (Flow cytometry – Syrian hamster). Diamond MS, Kinder M, Matsushita H, Mashayekhi M, Dunn GP, Archambault JM, Lee H, Arthur CD, White JM, Kalinke U, Murphy KM, and Schreiber RD. 2011. J. Exp. Med. 208: 1989-2003. (in vitro blocking) Kao H, Lin J, Littman DR, Shaw AS, and Allen PM. 2008. J. Immunol. 181: 8248-8257. (Immunoprecipitation) Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross M, suri A, and Allen PM. 2007. Nat. Immunol. 8:388-397. (in vitro blocking) |
Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.
Related Products
HOW CAN WE HELP YOU? Our specialists are to help you find the best product for your application. We will be happy to help you find the right product for the job.
TALK TO A SPECIALIST
Contact our Customer Care, Sales & Scientific Assistance
EMAIL US
Consult and asked questions about our products & services
DOCUMENTATION
Documentation of Technical & Safety Data Sheet, Guides and more..
NEWSLETTER
We gladly support you by keeping you updated on our latest products and the developments around our services.
ATLANTIS BIOSCIENCE PTE LTD
362 Upper Paya Lebar Rd, #07-15,
Singapore 534963
WeChat ID: +65 8608 0974
© 2015-2023 Atlantis Bioscience Pte Ltd. All rights reserved. Co Reg No: 201539516N