Product Description
A visible, blue protein gel staining solution. Fast protein staining in a single step without fixation or washing.
Product Description
One-Step Blue® Protein Gel Stain is a ready-to-use visible blue protein gel staining solution. With no fixation or wash steps required, One-Step Blue® is a great choice for convenience and safety. One-Step Blue® can detect as little as approximately 10-20 ng of protein per band.
- One-step staining procedure, no fixation required
- Non-toxic formulation is safe for easy disposal
- Sensitive protein detection down to 10-20 ng
- Visualize blue bands, or image with a near-infrared fluorescence imager
- 1 L size
- Store at 4°C
Fast and simple procedure
One-Step Blue® produces fast (5-60 min) protein staining in a single step without fixation or washing. Destaining is optional. One-Step Blue® has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins. The staining is fully compatible with mass spectrometry and Edman-based sequencing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence.
One-Step Blue® is non-toxic
One-Step Blue® offers safer handling and disposal compared to Coomassie staining because it is entirely aqueous-based, without hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.
![](https://www.atlantisbioscience.com/wp-content/uploads/2023/10/One-Step-Blue.png)
Please visit our Protein Gel Stains technology page to learn about our other safe and sensitive protein gel stains such as One-Step Lumitein™ and One-Step Blue™, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue staining.
Product | Replacement for | Catalog number | Unit size | Product protocol | Safety report |
---|---|---|---|---|---|
One-Step Blue® | Coomassie stain | 21003-1L | 1L | PI-21003 | One-Step Gel Stains Safety Report |
One-Step Lumitein™ | SYPRO® Ruby Protein Gel Stain | 21004-1L | 1L | PI-21004 | |
21004-4L | 4L | ||||
One-Step Lumitein™ UV | Oriole™ and Flamingo™ Protein Gel Stains | 21005-1L | 1L | PI-21005 | |
21005-4L | 4L |
One-Step Blue is a registered trademark of Biotium. Lumitein and its related technologies are covered by US and international patents. SYPRO is a registered trademark of Molecular Probes, Inc. Oriole and Flamingo are trademarks of Bio-Rad Laboratories. Cubitainer is a registered trademark of Hedwin Corporation.
Protocols
SDS
Reference Publications
Antonio Barreiro, Antoni Prenafeta, Gregori Bech-Sabat, Mercè Roca, Eva Perozo Mur, et al.
Preclinical evaluation of a COVID-19 vaccine candidate based on a recombinant RBD fusion heterodimer of SARS-CoV-2
bioRxiv 2021.11.22.469117;
DOI: https://doi.org/10.1101/2021.11.22.469117
Article Snippet: “The purified antigen was then buffer exchanged by tangential flow filtration and filter sterilised. Purity and integrity were evaluated by SDS-PAGE with Bolt™ 4 to 12% Bis-Tris gels (Thermo Fisher, ref. NW04120BOX), stained with One-Step Blue Protein Gel Stain (Biotium, ref. 21003).”
Guisheng Zeng, Xiaoli Xu, Yee Jiun Kok, et al.
Cytochrome c regulates hyphal morphogenesis by interfering with cAMP-PKA signaling in Candida albicans
Cell Reports 42, 113473
DOI: https://doi.org/10.1016/j.celrep.2023.113473
Article Snippet: Highlight from the Chemicals, peptides, and recombinant proteins table “One-Step blue protein gel stain Biotium Cat#21003.”
Luo, Huacheng, et al.
HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia
Molecular cell vol. 82,4 (2022): 833-851.e11.
DOI: 10.1016/j.molcel.2022.01.014
Article Snippet: Highlight from the Chemicals, Peptides, and Recombinant Proteins table “BrUTP Biotium Cat#40026, One-Step Blue® Protein Gel Stain Biotium Cat#21003.”