CRISPR/Cas9-rAAV Vectors – Genome Editing Research
Our pre-made CRISPR/Cas9 AAV vectors provide a powerful and efficient tool for precise genome editing. Designed to streamline your research workflow, these vectors offer a range of features to meet your specific needs.
Key Features:
High-Efficiency Cas9 Expression: Our vectors deliver robust Cas9 expression, ensuring efficient gene editing.
Versatile Serotypes: Choose from a variety of serotypes to target specific tissues and cell types.
Flexible Promoters: Select from a range of promoters to control gene expression levels.
Rapid Turnaround: Our pre-made vectors are available for immediate shipping, accelerating your research timeline.
High–Quality Production: Rigorously tested and quality-controlled to ensure optimal performance.
Applications:
- Gene Knockout: Precisely disrupt gene function to study gene loss-of-function phenotypes.
- Gene Knock-In: Introduce specific genetic modifications, such as point mutations or insertions.
- Gene Activation and Repression: Modulate gene expression levels using CRISPR-based transcriptional activators and repressors.
- Genome Engineering: Create complex genetic modifications, including large deletions, inversions, and translocations.
Simplify Your Workflow, Maximize Your Impact
Our pre-made CRISPR/Cas9 AAV vectors are designed to simplify your genome editing experiments. By eliminating the need for time-consuming vector construction and production, you can focus on your core research objectives.
Contact us today to discuss your research goals and explore our range of pre-made and custom CRISPR/Cas9 AAV vectors.
Product Specification
Promoter type | Catalog No. | Promoter and Gene | |||
Universal promoter | AAV-XA20 | ssAAV.miniCMV.SpCas9 | |||
AAV-XA02 | ssAAV.EFS.SpCas9 | ||||
AAV-XB02 | ssAAV.EFS.SpCas9HF | ||||
AAV-XB06 | ssAAV.miniCMV.SpCas9HF | ||||
AAV-AC010 | ssAAV.EFS.SaCas9 | ||||
AAV-AC040 | ssAAV.CMV.SaCas9 | ||||
AAV-AD040 | ssAAV.CMV.SaCas9HF | ||||
AAV-AD010 | ssAAV.EFS.SaCas9HF | ||||
AAV-XE01G | ssAAV.EFS.AsCpfl | ||||
AAV-XE01H | ssAAV.EFS.LbCpfl | ||||
AAV-XE02H | ssAAV.miniCMV.LbCpfl | ||||
Tissue-specific promotor | AAV-AC050 | ssAAV.TBG.SaCas9 | |||
AAV-AC060 | ssAAV.Elastasel.SaCas9 | ||||
AAV-AC070 | ssAAV.Rat Insulin 2.SaCas9 | ||||
AAV-AC080 | ssAAV.mouse Insulin2.SaCas9 | ||||
AAV-AC090 | ssAAV.hDesmin.SaCas9 | ||||
AAV-AC100 | ssAAV.hSynapsin.SaCas9 | ||||
AAV-AC120 | ssAAV.pMECP2.SaCas9 | ||||
AAV-AC190 | ssAAV.CBh.Sacas9.WPRE.SV40pA | ||||
AAV-AC071 | ssAAV.Rat Insulin 2.SaCas9.U6.(Sa)sgRNA | ||||
AAV-AC081 | ssAAV.mouse Insulin2.SaCas9.U6.(Sa)sgRNA | ||||
AAV-AC091 | ssAAV.hDesmin.SaCas9.U6.(Sa)sgRNA | ||||
AAV-AC101 | ssAAV.hSynapsin.SaCas9.U6.(Sa)sgRNA | ||||
AAV-AC121 | ssAAV.pMECP2.SaCas9.U6.(Sa)sgRNA | ||||
AAV-AD060 | ssAAV.Elastasel.SaCas9HF | ||||
AAV-AD070 | ssAAV.rlnsulin2.SaCas9HF | ||||
AAV-AD080 | ssAAV.mlnsulin2.SaCas9HF | ||||
AAV-AD090 | ssAAV.hDesmin.SaCas9HF | ||||
AAV-AD100 | ssAAV.hSynapsin.SaCas9HF | ||||
AAV-AD120 | ssAAV.MECP2.SaCas9HF |
Case Study
Products:Â CRISPR AAV vectors from PackGene Biotech
Injection Method:Â Intramuscular Injection
Recommended Dose: 25 μL, 1.5E+12 vg/ml
Targeting Site:Â muscle
Animal Model:Â DMD mouse model
Journal:Â Genome Medicine, 2021 (IF=15.266)
Paper Title:Â In vivo genome editing in mouse restores dystrophin expression in Duchenne muscular dystrophy patient muscle fibers
DOI:Â 10.1186/s13073-021-00876-0
AAV production and injection
CRISPR AAV vectors were generated by PackGene Biotech Co. For intramuscular injection into NSI mice, the animals were anesthetized by abdominal injection of 5% chloral hydrate, followed by
injection of CRISPR AAV vector (25 μL, 1.5E+12 vg) into the TA muscle transplanted with DMD–MDSCs.