Product Description

High-Quality Classical Media for Optimal Cell Culture

Partnering with Biowest, we offer a comprehensive selection of trusted media formulations, including Dulbecco’s Modified Eagle Medium (DMEM), Eagle’s Minimum Essential Medium (EMEM), RPMI 1640, and Ham’s F-12. Our products are meticulously manufactured in state-of-the-art ISO certified facilities to ensure consistent quality and reliability.

Key Features:

• High Glucose w/ L-Glutamine w/ Sodium Pyruvate
• Versatile formulations: Choose from a variety of classical media to suit your specific cell culture needs.
• Proven performance: Compatible with a wide range of cell lines, including those from ATCC, DSMZ, and Riken.
• Exceptional quality: Manufactured in ISO 9001:2008 and ISO 13485:2012 certified facilities.
• Extended shelf life: Sterile-filtered media with a 12-month shelf life for convenience.
• Trusted by leading institutions: Widely used in renowned research centers like NUS and CSI in Singapore.

** please inquiry for powdered formulation

Please enquire with us at [email protected]

Reference Publications

Ginting, C. N., Lister, I. N. E., Girsang, E., Mutia, M. S., Lubis, Y. E. P., Amalia, A., Rizal, R., & Widowati, W.

Anti-inflammatory Activity of Quercitrin on Hypoxia-induced EA.hy926

Journal of Physics Conference Series, 1374(1), 012033 (2019).

DOI: https://doi.org/10.1088/1742-6596/1374/1/012033

Article Snippet: “The cell lines were grown in Dulbecco’s Modified Eagle Medium
DMEM high glucose (Biowest L0104) supplemented with 10% fetal bovine serum (FBS) (Biowest
S181G), 1% antibiotic-antimycotic 100x (Biowest L0010), 1% Amphotericin B 100x (Biowest L0009).”

Sri Nadya Saanin, Roro Wahyudianingsih, Merry Afni, Ervi Afifah, Maesaroh Maesaroh and Wahyu Widowati

Suppression of pro-inflammatory cytokines and mediators production by ginger
(Zingiber officinale Roscoe) ethanolic extract and gingerol in lipopolysaccharideinduced RAW 264.7 murine macrophage cells

Indian Journal of Natural Products and Resources Vol. 11(4), December 2020, pp. 260-266

http://nopr.niscpr.res.in/handle/123456789/56146

Article Snippet: “The cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Biowest, L0104) supplemented with 20% Fetal Bovine Serum (FBS) (Biowest, S1810), 1% Penicillin-Streptomycin (Penstrep) (Biowest, L0022).”

Ginting, Chrismis Novalinda; Lister, I Nyoman E.; Girsang, Ermi; Artie, Dwi Surya; Aviani, Jenifer Kiem; Widowati, Wahyu.

Apoptotic, MDA, and FGF2 Level of Quercitrin Treatment on Hypoxic-Induced EA.hy926 Cell Line.

Journal of Reports in Pharmaceutical Sciences 10(1):p 22-28, Jan–Jun 2021.

DOI: https://doi.org/10.4103/jrptps.JRPTPS_70_20

Article Snippet: “The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Biowest, L0104-500) supplemented with 10% fetal bovine serum (FBS) (Biowest, S181-500), 1% nanomycopulitine (Biowest, L-X16-100), 1% antibiotic/antimycotic (ABAM) (Biowest, L0010-100), 1% Amphotericin B (Biowest, L0009-050), and 0,1% Gentamicin (Gibco, 15750060), respectively. The cells were incubated at 37^oC with 5% CO₂.”

Jorratt, P., Ricny, J., Leibold, C. et al.

Endogenous Modulators of NMDA Receptor Control Dendritic Field Expansion of Cortical Neurons.

Mol Neurobiol 60, 1440–1452 (2023).

DOI: https://doi.org/10.1007/s12035-022-03147-0

Article Snippet: “After 3 washes in HBSS, tissue was dissociated mechanically using a 0.9-mm needle (B. Braun, 4657519) followed by a 0.45-mm needle (B. Braun, 4657683). The tissue suspension was subsequently passed through a 100-μm pore nylon filter (VWR, 732–2759) and re-suspended in a seeding medium, which contains DMEM (Biowest, L0104-500) supplemented with 10% FBS (Biowest, S1810-500)…”

Widowati, W., Murti, H., Widyastuti, H., Laksmitawati, D. R., Rizal, R., Widya Kusuma, H. S., Sumitro, S. B., Widodo, M. A., & Bachtiar, I.

Decreased Inhibition of Proliferation and Induction of Apoptosis in Breast Cancer Cell Lines (T47D and MCF7) from Treatment with Conditioned Medium Derived from Hypoxia-Treated Wharton’s Jelly MSCs Compared with Normoxia-Treated MSCs.

International journal of hematology-oncology and stem cell research, 15(2), 77–89. (2021).

DOI: https://doi.org/10.18502/ijhoscr.v15i2.6038

Article Snippet: “The medium consists of Dulbecco’s modified Eagle’s medium (DMEM, Biowest, L0104) for MCF7 and Roswell Park Memorial Institute medium (RPMI 1640, Biowest, L0495) for T47D. FBS 10% (Biowest, S181H) and 1% penicillin-streptomycin (Biowest, L0018) were then incubated (21% O₂, 5% CO₂, 37 ℃).”

Terzopoulou Z, Michopoulou A, Palamidi A, Koliakou E, Bikiaris D.

Preparation and Evaluation of Collagen-Based Patches as Curcumin Carriers.

Polymers. 2020; 12(10):2393.

DOI: https://doi.org/10.3390/polym12102393

Article Snippet: “For the isolation of the fibroblasts, the pieces of the dermis were placed in culture in DMEM with L-glutamine (BIOWEST, #L0104-500) supplemented with 10% fetal bovine serum (FBS) and penicillin–streptomycin (BIOWEST, # L0018-100) and maintained at 37 °C in a humified atmosphere containing 5% CO₂“

Gupta, S., Kumar, P., Rathi, B. et al.

Targeting of Uropathogenic Escherichia coli papG gene using CRISPR-dot nanocomplex reduced virulence of UPEC.

Sci Rep 11, 17801 (2021).

DOI: https://doi.org/10.1038/s41598-021-97224-4

Article Snippet: “HeLa cells were cultured in Dulbecco’s Modified Eagle Medium (high glucose) supplemented with 10% heat inactivated fetal bovine serum (cat#L0104, Biowest, Nuaille, France) and incubated at 37 °C and 5% CO₂ in a humidified incubator.”

Piotr Celichowski, Marcello Turi, Sandra Charvátová, Dhwani Radhakrishnan, Neda Feizi, Zuzana Chyra, Michal Šimíček, Tomáš Jelínek, Juli Rodriguez Bago, Roman Hájek & Matouš Hrdinka.

Tuning CARs: recent advances in modulating chimeric antigen receptor (CAR) T cell activity for improved safety, efficacy, and flexibility.

Journal of Translational Medicine 21:1. (2023)

DOI: https://doi.org/10.1080/2162402X.2022.2140534

Article Snippet: “HEK293T cells were cultured in Dulbecco’s modified Eagle medium (Biowest, catalog no.: L0104) supplemented with 10% FBS.”

Hanna Sari Widya Kusuma, Hartini Tiono, Philips Onggowidjaja, Selonan Susang Obeng, Wahyu Widowati, Cintani Dewi Wahyuni, Cahyaning Riski Wijayanti, Muhamad Aldi Maulana, Tri Handayani and Rizal Rizal

Anti-inflammatory Activities of Pineapple (Ananas comosus) Core Extract in Lipopolysaccharide-induced RAW264.7 Cell Line

DOI: https://doi.org/10.5220/0010744300003113

Article Snippet: “RAW 264.7 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Biowest, L0104) supplemented with 10% fetal bovine serum (FBS) (Biowest, S1810)…”

Documents

Data Sheet

DMEM High Glucose w/ L-Glutamine w/ Sodium Pyruvate L0104_MSDS

DMEM High Glucose w/ L-Glutamine w/ Sodium Pyruvate L0104_Technical Data Sheet

DMEM High Glucose w/ L-Glutamine w/ Sodium Pyruvate L0104_Composition