OMEGA96-ACE
Triple-Chamber Neuronal Co-Culture Microplate

1 x OMEGA^96-ACE screening plate, 32 experiments! Equivalent to 32 OMEGA^ACE or OMEGA^ACE-2mm devices, at a fraction of the price!

The first of its kind. It is the scaled up version of the OMEGA^ACE and High-Throughput Screening (HTS) compatible.

Speed and scalability are the key to rapidly identifying new therapeutics. Owing to the unique open-top chamber design, the OMEGA^96-ACE plate is the ONLY triple-chamber microfluidic platform commercially available that fully conforms to the 96-well microplate standard. As such, the OMEGA^96-ACE plate is compatible with most lab automation and robotic systems such as microplate washers, plate readers, high-content imagers, pinning tools and plate hotels.

OMEGA^96-ACE screening plate can be used for in vitro triple co-cultures and studying neuronal propagation.

OMEGA^96-ACE plate features:

• Ready-to-use: sterile and pre-bonded to a high-transmissive glass coverslip
• Optionally  pre-coated with either poly-D-lysine or poly-L-ornithine
  
• 32 experiments can be performed per plate – perfect for screening!
• Open-top chamber design provides full access to your cultures – use explants or spheroids in your experiment

Just starting out? Then you might want to try our OMEGA^ACE or OMEGA^ACE-2mm device first.

Need only two culture chambers? The OMEGA⁹⁶ might be what you are looking for!

Description

The OMEGA series of microfluidic devices from eNUVIO are perfectly suited for experiments involving neuronal co-culture and compartmentalization. Each of the growth chambers of the device are physically separated by a series of microfluidic channels that serve to fluidically isolate each of the adjoined chambers. These microchannels are large enough however, to allow for neurites/axons to project into and interact with cultures located in the adjacent chamber. OMEGA^96-ACE devices each contain three serially-adjoined culture chambers. Compared to our two-chamber devices, triple-chamber devices provide researchers with the ability to perform more specialized co-culture and compartmentalization experiments.

The unique open-chamber design of OMEGA series devices gives scientists direct access to their cultures, enabling researchers to explore new types of experimental paradigms not possible with standard closed-chamber devices.  For example, the chambers are large enough to house tissues that are several millimeters in diameter, accommodating tissue explants or self-assembled 3D cultures (e.g. spheroids, organoids). Additionally, conventional patch-clamp electrodes can openly access the cultures, allowing researchers to examine the electrophysiological properties of the culture.

OMEGA^96-ACE plates are shipped ready to use. They are sterile, pre-bonded to high-transmissive glass, and optionally pre-coated with either poly-D-lysine or poly-L-ornithine. Maintaining healthy cultures over a long period of time is even easier with OMEGA^96-ACE plates, owing to the evaporation-minimizing tracks around the edge of each plate. 

OMEGA^96-ACE devices can be used to set up in vitro triple co-cultures and study neuronal propagation.

Culture compatibility:

• iPSC-derived cultures
• Self-assembled 3D cultures (e.g. spheroids, organoids)
• Primary cultures
• Explant tissues
• Immortalized cell lines

OMEGA^96-ACE specifications:

• 32 well-pairs per microplate = 32 individual experiments per plate
• Microplate dimensions (L x W x H): 127.2 x 85.3 x 14.1 mm
• Chamber #1 and #3 dimensions (L x W x H): 7 x 6 x 13 mm
• Chamber #2 dimensions (L x W x H): 8 x 6 x 13 mm
• Chamber pitch: 9 mm
• Microchannel width: 10 μm
• Microchannel length: ~640 μm
• Microchannels/chamber pair: 70
• Pre-bonded glass bottom: 200 μm thick
• Shipped sterile in PBS, ready to use
• Hydrophilic, no need for plasma treatment
• Option: pre-coated with poly-D-lysine or poly-L-ornithine
• Anti-evaporation tracks around the edge of each plate