Q：If there are cells existing in the channel, will it affect the monoclonality of my single cell-derived colony? If so, how do I perform the subsequent cell harvesting steps to avoid contaminating my single cell colonies?

• Cells that exist in the channel do have the potential to contaminate your single-cell colonies and affect monoclonality. So, It’s important to ensure that the washing step is done well to remove excess cells from the chip.If residual cells still exist in the channel, do not directly inject trypsin into the chip before the harvesting stage. Instead, disassemble the chip while the cells are still attached, then individually add trypsin to each culture well to detach the cells. The working volume of each culture well is 1.5 μL.

Q：Following the previous question, what do you mean by high single cell growth rate? In theory, single cells don’t grow into colonies efficiently, why is this?

• Because all the cells inside the CellGem® chip share the same pool of culture medium and also share secreted growth factors between each other. Each single cell still owns its isolated growth space at the same time.(Depend on cell type.)

Q: Is there a contamination risk of using sealing tape to seal the chip inlets?

• The sealing tape used in the kit is tested and certified to be sterile. It’s safe to use with cell culture.